Cloning and expression of a novel zinc finger gene, Fez, transcribed in the forebrain of Xenopus and mouse embryos
Cloning and expression of a novel zinc finger gene, Fez, transcribed in the forebrain of Xenopus and mouse embryos
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DOI：
10.1016/s0925-4773(00)00264-1
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发表时间：
2000-05-01
影响因子：
2.6
通讯作者：
Sargent, TD
中科院分区：
文献类型：
--
作者：
Matsuo-Takasaki, M;Lim, JH;Sargent, TD
我们已经确定并克隆了一个新的锌指基因，Fez（前脑胚胎锌指），作为一个潜在的下游决定因素的前神经板形成在非洲爪蟾。Fez作为由神经化因子noggin诱导的几种神经特异性基因之一被分离（Smith和Harland，1992. Cell 70，829-840），在未定型外胚层中。Fez具有一个由466个氨基酸组成的开放阅读框架，并含有6个C2 H2型锌指结构域，这些结构域在果蝇、斑马鱼、小鼠和人中高度保守。在非洲爪蟾中，Fez的表达开始于阶段12的神经板的吻端，到阶段45，它定位于几个端脑区域，包括嗅球、终神经和脑室区。Fez的小鼠同源物在胚胎第11天的小鼠前脑中也有类似的表达。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
We have identified and cloned a novel zinc finger gene, Fez (forebrain embryonic zinc-finger), as a potential downstream determinant of anterior neural plate formation in Xenopus. Fez was isolated as one of several neural-specific genes that was induced by the neuralizing factor, noggin (Smith and Harland, 1992. Cell 70, 829-840), in uncommitted ectoderm. Fez has an open reading frame comprising 466 amino acids, and contains six C2H2 type zinc finger domains, which are highly conserved among Drosophila, zebrafish, mouse, and human. In Xenopus, the expression of Fez begins at stage 12 in the rostral end of the neural plate, and by stage 45, it is localized to several telencephalic regions, including the olfactory bulbs, nervus terminalis, and ventricular zone. The mouse homologue of Fez is similarly expressed in the mouse forebrain by embryonic day 11. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.