Identification of neonatal rat hippocampal radial glia cells in vitro
Identification of neonatal rat hippocampal radial glia cells in vitro
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新生大鼠海马放射状胶质细胞的体外鉴定
DOI：
10.1016/j.neulet.2010.12.054
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发表时间：
2011-03-03
影响因子：
2.5
通讯作者：
Zou, Linqing
中科院分区：
文献类型：
--
作者：
Li, Haoming;Jin, Guohua;Zou, Linqing
放射状胶质细胞（RGCs）作为神经祖细胞和迁移神经元的指南的作用是公认的，而他们的确切贡献成人海马神经发生仍然不太清楚。为了更准确地研究正常条件下体外培养的海马RGCs的特性，本研究在贴壁条件下体外培养了出生后1 d的大鼠海马RGCs，鉴定了其星形胶质细胞和干/祖细胞特性。结果发现，新生大鼠海马RGC比胎鼠大脑皮质RGC突起更长，能被BLBP、GFAP、Vimentin和Nestin双标记，表达干/祖细胞基因，具有多向分化潜能，但在体外正常培养条件下，其胶质细胞生成能力远远超过神经细胞生成能力。综上所述，我们在体外获得并鉴定了新生大鼠视网膜神经节细胞的一些特性。(C)2010爱思唯尔爱尔兰有限公司版权所有。
The role of radial glia cells (RGCs) as neural progenitors and as guides for migrating neurons is well established, whereas their precise contribution to adult hippocampal neurogenesis remains less understood. To precisely study the properties of hippocampal RGCs under normal conditions in vitro, here we acquired the hippocampal RGCs of postnatal 1 d rats under adherent conditions in vitro, identified their astroglia and stem/progenitor properties. We found that the neonatal rat hippocampal RGCs had longer processes than the RGCs from fetal cerebral cortices, and these cells could be double-labeled by BLBP, GFAP, Vimentin with Nestin and expressed some stem/progenitor genes, these cells also presented multiple differentiation potentialities, albeit the ability of gliogenesis far exceeded the neurogenesis under normal culture conditions in vitro. Taken together, we acquired and identified some properties of the RGCs from neonatal rat hippocampi in vitro. (C) 2010 Elsevier Ireland Ltd. All rights reserved.