Betanin-enriched red beet extract attenuated platelet activation and aggregation by suppressing Akt and P38 Mitogen-activated protein kinases phosphorylation
Betanin-enriched red beet extract attenuated platelet activation and aggregation by suppressing Akt and P38 Mitogen-activated protein kinases phosphorylation
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富含甜菜苷的红甜菜提取物通过抑制 Akt 和 P38 丝裂原激活蛋白激酶磷酸化来减弱血小板活化和聚集
DOI：
10.1016/j.jff.2019.103491
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发表时间：
2019-10-01
影响因子：
5.6
通讯作者：
Yang, Yan
中科院分区：
文献类型：
--
作者：
Song, Fenglin;Zuo, Xiao;Yang, Yan
甜菜碱是一种水溶性含氮色素，具有抗氧化、抗炎、降血糖和心脏保护等多种保健作用。然而，甜菜素在血小板功能中的作用是完全未知的。在本研究中，我们研究了富含甜菜碱的红甜菜提取物对体外人血小板生理的影响，并探讨了其作用机制。结果表明，甜菜碱可剂量依赖性地降低血小板P-选择素、CD63、CD40L的表达和PAC-1结合率，抑制ATP分泌和血小板聚集。同时，甜菜碱不能延长C57BL/6小鼠的尾部出血时间。Western印迹结果显示，甜菜碱能抑制凝血酶诱导的血小板Akt和P38MAPK的磷酸化。综上所述，甜菜素可以在不影响出血时间的情况下减弱血小板功能，这种作用可能是通过抑制Akt和P38MAPK的磷酸化来实现的。我们的研究结果表明，甜菜素可以被认为是预防血小板相关血栓栓塞性心血管疾病的候选药物。
Betanin, a water-soluble nitrogenous pigment, is found to exert various health benefits including anti-oxidative, anti-inflammatory, anti-hyperglycemic and cardioprotective effects. However, the roles of betanin in platelet function is completely unknown. In the present study we investigated the effects of betanin-enriched red beet extract on human platelet physiology in vitro and explored the underlying mechanism. The results showed that betanin significantly and dose-dependently reduced platelet P-selectin, CD63, CD40L expression and PAC-1 binding, inhibited ATP secretion and platelet aggregation. Meanwhile, betanin didn't prolong tail bleeding time in C57BL/6 mice. Western blot results demonstrated that betanin attenuated platelet Akt and P38 MAPK phosphorylation induced by thrombin. Taken together, betanin could attenuate platelet function without affecting bleeding time, and this effect is potentially mediated by inhibition of Akt and P38 MAPK phosphorylation. Our findings suggest that betanin could be considered as a candidate agent for the prevention of platelet associated thromboembolic CVDs.