The expression profile of angiotensin system on thawed murine ovaries
The expression profile of angiotensin system on thawed murine ovaries
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DOI：
10.1007/s13770-016-0009-y
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发表时间：
2016-12-01
影响因子：
3.6
通讯作者：
Ku, Seung-Yup
中科院分区：
文献类型：
--
作者：
Kim, Yoon Young;Kim, Yong Jin;Ku, Seung-Yup
冻融法是目前应用最广泛的组织工程卵巢保存技术之一。许多细胞和组织在解冻后表现出功能基因表达的变化。血管紧张素（AT）系统在卵巢卵泡发育过程中起重要作用。AT系统由ATII、ATII Ⅰ型受体（ATII-RI）和ATII Ⅱ型受体（ATII-RII）组成。然而，很少有人知道是否冻融卵巢显示任何改变AT系统成员的基因表达时，与生存增强因子。我们的目的是调查是否大量冷冻和解冻与或不使用Rho相关激酶（ROCK）抑制剂上调或下调ATII，ATII-RI，ATII-RII基因的表达冷冻解冻卵巢组织。与新鲜对照相比，在解冻卵巢上观察到ATII、ATII-RI和ATII-RII基因表达的显著变化。用ROCK抑制剂处理没有显著改变它们的表达。因此，卵巢组织冻融后AT系统基因的mRNA表达水平可能受到影响，ROCK抑制剂活性的调节可能不影响冻融后卵巢组织AT系统的表达。
Freezing and thawing is one of the most widely used tissue engineering techniques for the preservation of ovaries. Many cells and tissues demonstrate changes in functional gene expression after thawing. Several studies have reported the important roles of angiotensin (AT) system during the ovarian follicular growth. AT system consists of ATII, and ATII receptors type I (ATII-RI) and type II (ATII-RII). However, little is known whether frozen-thawed ovaries show any alteration of AT system member gene expression when treated with survival-enhancing factors. We aimed to investigate whether mass freezing and thawing with or without the use of Rho-associated kinase (ROCK) inhibitors up- or down-regulate the expression of ATII, ATII-RI, and ATII-RII genes on frozen-thawed ovarian tissues. Significant changes in the expression of ATII, ATII-RI, and ATII-RII genes were observed on thawed ovaries when compared to fresh control. The treatment with ROCK inhibitors did not significantly alter their expression. In conclusion, freezing and thawing of ovarian tissue may affect the mRNA expression levels of intra-ovarian AT system genes, and modulation of ROCK inhibitor activity may not regulate AT system on the frozenthawed ovarian tissue.