To What Extent is FAIMS Beneficial in the Analysis of Proteins?
To What Extent is FAIMS Beneficial in the Analysis of Proteins?
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DOI：
10.1007/s13361-015-1326-4
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发表时间：
2016-04
影响因子：
3.2
通讯作者：
Cooper HJ
中科院分区：
文献类型：
--
作者：
Cooper HJ
高场不对称波形离子迁移率谱(FAIMS)，又称微分离子迁移率谱，是一种新兴的生物分子分析工具。本文讨论了FAIMS在蛋白质分析中的优点和局限性。阐述了FAIMS分离离子的原理和机理，并详细介绍了FAIMS与传统离子迁移率光谱的区别。蛋白质分析是从自上而下(完整蛋白质)和自下而上(蛋白水解肽)的角度来考虑的。评估了FAIMS在分析多个完整蛋白质的复杂混合物和分析单一蛋白质的多个构象中的作用。类似地，FAIMS在蛋白质组学和多肽的靶向分析中的应用也被考虑。ᅟ
High field asymmetric waveform ion mobility spectrometry (FAIMS), also known as differential ion mobility spectrometry, is emerging as a tool for biomolecular analysis. In this article, the benefits and limitations of FAIMS for protein analysis are discussed. The principles and mechanisms of FAIMS separation of ions are described, and the differences between FAIMS and conventional ion mobility spectrometry are detailed. Protein analysis is considered from both the top-down (intact proteins) and the bottom-up (proteolytic peptides) perspective. The roles of FAIMS in the analysis of complex mixtures of multiple intact proteins and in the analysis of multiple conformers of a single protein are assessed. Similarly, the application of FAIMS in proteomics and targeted analysis of peptides are considered. ᅟ