Emergence of a node-like population within an in vitro derived Neural Mesodermal Progenitors (NMPs) population
Emergence of a node-like population within an in vitro derived Neural Mesodermal Progenitors (NMPs) population
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DOI：
10.1101/326371
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发表时间：
2018-05
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影响因子：
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通讯作者：
Shlomit Edri;P. Hayward;W. Jawaid;A. M. Arias
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作者：
Shlomit Edri;P. Hayward;W. Jawaid;A. M. Arias
哺乳动物胚胎的尾侧外胚层（CLE）含有双能祖细胞，称为神经中胚层祖细胞（NMPs），其在整个轴向伸长过程中对脊髓和近轴中胚层起作用。在这里，我们进行了一个单细胞分析的不同的在体外的NMPs的人口产生的胚胎干细胞（ESC）或外胚层干细胞（EpiSC），并比较它们的E8.25 CLE小鼠胚胎。在我们对该区域的分析中，我们的发现挑战了NMPs应该共表达Sox 2和T的概念。我们建立了一个支持向量机（SVM）的基础上的胚胎CLE，并使用它作为一个分类模型来分析在体外NMP样群体。我们发现，ESC衍生的NMP是异质的，并且含有很少的NMP样细胞，而EpiSC衍生的NMP产生高比例的具有胚胎NMP特征的细胞。重要的是，我们发现在培养物中衍生Epi-NMPs的群体含有一个nodelike群体，该群体负责维持T在体外的表达。这些结果模拟了体内的事件，并提示了NMP出现的事件序列。
The mammalian embryos Caudal Lateral Epiblast (CLE) harbours bipotent progenitors, called Neural Mesodermal Progenitors (NMPs), that contribute to the spinal cord and the paraxial mesoderm throughout axial elongation. Here we performed a single cell analysis of different in vitro NMPs populations produced either from embryonic stem cells (ESCs) or epiblast stem cells (EpiSCs) and compared them to E8.25 CLE mouse embryos. In our analysis of this region our findings challenge the notion that NMPs should coexpress Sox2 and T. We built a Support Vector Machine (SVM) based on the embryo CLE and use it as a classification model to analyse the in vitro NMP-like populations. We showed that ESCs derived NMPs are heterogeneous and contain few NMP-like cells, whereas EpiSCs derived NMPs, produce a high proportion of cells with the embryo NMP signature. Importantly, we found that the population from which the Epi-NMPs are derived in culture, contains a nodelike population, which is responsible for maintaining the expression of T in vitro. These results mimic the events in vivo and suggest a sequence of events for the NMPs emergence.