Transcriptome sequencing revealed that knocking down FOXL2 affected cell proliferation, the cell cycle, and DNA replication in chicken pre-ovulatory follicle cells
Transcriptome sequencing revealed that knocking down FOXL2 affected cell proliferation, the cell cycle, and DNA replication in chicken pre-ovulatory follicle cells
复制标题
转录组测序显示，敲除 FOXL2 会影响鸡排卵前卵泡细胞的细胞增殖、细胞周期和 DNA 复制
DOI：
10.1371/journal.pone.0234795
复制
发表时间：
2020-07-09
期刊：
影响因子：
3.7
通讯作者：
Gong, Yanzhang
中科院分区：
文献类型：
--
作者：
Luo, Wei;Gu, Lantao;Gong, Yanzhang
叉头盒L2（FOXL2）是一个编码叉头转录因子的单外显子基因，主要在卵巢、眼睑和垂体中表达，在卵巢发育中起重要作用。为了揭示FOXL2对卵巢颗粒细胞生物学过程和基因表达的影响，我们建立了稳定的FOXL2敲除的卵巢颗粒细胞，并利用转录组测序技术对其进行了分析。结果表明，FOXL2基因敲低可影响细胞增殖、DNA复制和凋亡等生物学过程，影响细胞周期进程，促进细胞增殖和DNA复制，减少细胞凋亡，促进有丝分裂。此外，通过比较FOXL2基因敲除后的转录组，我们发现了一系列差异表达基因（DEG）和相关通路。这些结果表明，FOXL2可能通过介导这些基因和通路，诱导细胞增殖、周期和DNA复制，在卵巢发育和维持中发挥关键作用。
Forkhead box L2 (FOXL2) is a single-exon gene encoding a forkhead transcription factor, which is mainly expressed in the ovary, eyelids and the pituitary gland.FOXL2plays an essential role in ovarian development. To reveal the effects ofFOXL2on the biological process and gene expression of ovarian granulosa cells (GCs), we established stableFOXL2-knockdown GCs and then analysed them using transcriptome sequencing. It was observed that knocking downFOXL2affected the biological processes of cell proliferation, DNA replication, and apoptosis and affected cell cycle progression.FOXL2knockdown promoted cell proliferation and DNA replication, decreased cell apoptosis, and promoted mitosis. In addition, by comparing the transcriptome afterFOXL2knockdown, we found a series of DEGs (differentially expressed genes) and related pathways. These results indicated that, through mediating these genes and pathways, the FOXL2 might induce the cell proliferation, cycle, and DNA replication, and play a key role during ovarian development and maintenance.