The production of 'cell cortices' for light and electron microscopy
The production of 'cell cortices' for light and electron microscopy
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DOI：
10.1034/j.1600-0854.2000.010704.x
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发表时间：
2000-07-01
期刊：
影响因子：
4.5
通讯作者：
Heuser, J
中科院分区：
文献类型：
--
作者：
Heuser, J
细胞膜的内表面提供了丰富的结构信息，这些信息对于理解细胞皮层的行为，特别是整个细胞的行为至关重要。四分之一个世纪前，Mazia等人。(1)，Clarke et al. (2)，Vacquier（3）和他们在伯克利的同事介绍了一种强大的方法，通过光学和电子显微镜观察这个表面，这在原则上，保持不变，直到今天。他们是第一个意识到聚赖氨酸（和其他阳离子多肽）强烈吸附到各种固体表面，产生高密度的游离阳离子位点，通过与细胞表面上占主导地位的负电荷的静电相互作用强烈地“粘合”细胞。在他们最初的报告中，这些作者指出，这样附着在多聚赖氨酸表面上的细胞“仍然活着，但可能会变平或扩散到不正常的程度，但可能是观察目的所需的”。
The inner surface of the cell membrane offers a wealth of structural information that is vital for understanding the behavior of the cell cortex in particular, and the whole cell in general. A quarter of a century ago, Mazia et al.(1), Clarke et al.(2), Vacquier (3), and their colleagues at Berkeley introduced a powerful method for viewing this surface by light and electron microscopy, which, in principal, remains unchanged to this day. They were the first to realize that polylysine (and other cationic polypeptides) adsorb strongly to various solid surfaces creating a high density of free cationic sites that strongly ‘glue’cells down through electrostatic interactions with the negative charges that predominate on the cell surface. In their original report (1), these authors noted that cells thus attached to polylysine surfaces‘remain alive, but may flatten or spread themselves to a degree that is not normal but may be desirable for observational purposes.’