The role of basement membrane proteins on the expression of neural cell adhesion molecule (N-CAM) in an adenoid cystic carcinoma cell line
The role of basement membrane proteins on the expression of neural cell adhesion molecule (N-CAM) in an adenoid cystic carcinoma cell line
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DOI：
10.1016/s1368-8375(99)00087-1
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发表时间：
2000-03-01
期刊：
影响因子：
4.8
通讯作者：
Araújo, NS
中科院分区：
文献类型：
--
作者：
França, CM;Jaeger, MMM;Araújo, NS
我们研究了神经细胞粘附分子（N-CAM）在腺样囊性癌细胞系CAC 2使用免疫荧光显微镜的存在。此外，我们分析了层粘连蛋白和1V型胶原蛋白在N-CAM表达中的作用。我们证明培养的腺样囊性癌细胞表达N-CAM。对照组细胞膜上有散在的N-CAM表达。IV型胶原对N-CAM分布无影响。在层粘连蛋白包被的盖玻片上生长的CAC 2细胞表达集中在细胞板状伪足上的N-CAM，这表明与迁移活性的关系。我们的研究结果表明，培养的腺样囊性癌细胞表达N-CAM，这种表达受到细胞外基质的调节。(C)2000由爱思唯尔科学有限公司出版。版权所有。
We investigated the presence of neural cell adhesion molecule (N-CAM) in the adenoid cystic carcinoma cell line CAC2 using immunofluorescence microscopy. Additionally, we analysed the role of laminin and type 1V collagen in N-CAM expression. We demonstrated that cultured adenoid cystic carcinoma cells express N-CAM. Control cells presented a scattered N-CAM expression on cell membrane. and type IV collagen had no effect in N-CAM distribution. CAC2 cells grown on laminin-coated coverslips expressed N-CAM concentrated on cell lamellipodia suggesting relationship with migratory activity. Our results showed that cultured adenoid cystic carcinoma cells express N-CAM and this expression is modulated by extracellular matrix. (C) 2000 Published by Elsevier Science Ltd. All rights reserved.