NOTATIN - AN ANTI-BACTERIAL GLUCOSE-AERODEHYDROGENASE FROM PENICILLIUM-NOTATUM WESTLING AND PENICILLIUM-RESTICULOSUM SP-NOV
NOTATIN - AN ANTI-BACTERIAL GLUCOSE-AERODEHYDROGENASE FROM PENICILLIUM-NOTATUM WESTLING AND PENICILLIUM-RESTICULOSUM SP-NOV
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DOI：
10.1042/bj0390024
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发表时间：
1945-01-01
影响因子：
4.1
通讯作者：
BIRKINSHAW, JH
中科院分区：
文献类型：
--
作者：
COULTHARD, CE;MICHAELIS, R;BIRKINSHAW, JH
本文详细介绍了notatum中notatin的生产、分离和性质。Notatin是一种黄素蛋白性质的酶，通过大气O2催化葡萄糖氧化为葡萄糖酸，产生H2 O2。一个纯净的咖啡。颜色为浅黄色，在0[度]下至少稳定8个月，易溶于水，且不会通过硝酸纤维素膜。水溶液。pH值为6-7，在270/280、375/380和450/460 μ m处有最大吸收。它抑制葡萄糖肉汤中1：1十亿稀释度的金黄色葡萄球菌。这种活性在pH < 2 and >8或加热到60[度]时迅速被破坏。抗菌活性是由于在葡萄糖和O2存在下产生H2 O2。d-木糖和d-甘露糖在酶反应中起作用，但葡萄糖的活性更高。麦芽糖、阿拉伯糖和半乳糖不摄取O2。
The production, isolation and properties of notatin from P. notatum are described in detail. Notatin is an enzyme of flavoprotein nature, catalyzing the oxidation of glucose to gluconic acid by means of atmospheric O2 with production of H2O2. A purified prepn. was buff to yellow in color, stable for at least 8 months at 0[degree], freely soluble in water, and will not pass through a nitro-cellulose membrane. Aqueous solns. have a pH of 6-7, and exhibit absorption maxima at 270/280, 375/380 and 450/460 mu. It inhibits Staphylococcus aureus in glucose broth in dilutions of 1:1 billion. This activity is rapidly destroyed at pH < 2 and > 8, or on heating to 60[degree]. The antibacterial activity is due to the production of H2O2 in the presence of glucose and O2. d-Xylose and d-mannose can function in the en-zymic reaction but glucose shows a higher activity. Maltose, arabinose and galactose show no O2 uptake.