Molecular characterization and expression analysis of a gene encoding for farnesyl diphosphate synthase from Euphorbia pekinensis Rupr
Molecular characterization and expression analysis of a gene encoding for farnesyl diphosphate synthase from Euphorbia pekinensis Rupr
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大戟法尼基二磷酸合酶基因的分子表征和表达分析
DOI：
10.1007/s11033-011-0886-z
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发表时间：
2012-02-01
影响因子：
2.8
通讯作者：
Jiang, Jihong
中科院分区：
文献类型：
--
作者：
Cao, Xiaoying;Yin, Ting;Jiang, Jihong
大戟根作为传统的中草药，在中国药典中有治疗水肿、淋病、偏头痛和疣的记载。本研究报道了一个新的法呢基二磷酸合酶（FPS）基因的cDNA克隆和鉴定。北京种。该基因全长1431 bp，开放阅读框1029 bp，编码342个氨基酸。推导的EpFPS氨基酸序列与其它植物FPS具有高度的同源性，并含有5个保守结构域。系统发育分析表明，EpFPS属于植物FPS类群。Southern杂交分析表明，在大肠杆菌中存在一个FPS基因小家族。北京种。表达谱分析表明，EpFPS在根中表达量较高，在叶和茎中表达量较低。在愈伤组织中，茉莉酸甲酯对EpFPS基因的表达和三萜类化合物的合成有强烈的诱导作用，而水杨酸对EpFPS基因的表达和三萜类化合物的合成有轻微的诱导作用。麦角固醇营养缺陷型酵母菌株中EpFPS的功能互补表明，克隆的cDNA编码功能性法呢基二磷酸合酶。
The root of Euphorbia pekinensis as a traditional herbal medicine has been recorded in Chinese pharmacopoeias for the treatment of oedema, gonorrhea, migraine and wart cures. In this work, we reported on the cDNA cloning and characterization of a novel farnesyl diphosphate synthase (FPS) from E. pekinensis. The full-length cDNA named EpFPS (Genbank Accession Number FJ755465) contained 1431 bp with an open reading frame of 1029 bp encoding a polypeptie of 342 amino acids. The deduced amino acid sequence of the EpFPS named EpFPS exhibited a high homology with other plant FPSs, and contained five conserved domains. Phylogenetic analysis showed that EpFPS belonged to the plant FPS group. Southern blot analysis revealed that there exists a small FPS gene family in E. pekinensis. Expression pattern analysis revealed that EpFPS expressed strongly in root, weak in leaf and stem. In callus, expression of EpFPS gene and biosynthesis of triterpenoids were strongly induced by Methyl jasmonate and slightly induced by Salicylic acid. Functional complementation of EpFPS in an ergosterol auxotrophic yeast strain indicated that the cloned cDNA encoded a functional farnesyl diphosphate synthase.