Extended Release of an Anti-Heparan Sulfate Peptide From a Contact Lens Suppresses Corneal Herpes Simplex Virus-1 Infection.
Extended Release of an Anti-Heparan Sulfate Peptide From a Contact Lens Suppresses Corneal Herpes Simplex Virus-1 Infection.
复制标题
从隐形眼镜延长抗甲烷硫酸盐肽的释放会抑制角膜疱疹单纯疱疹病毒-1感染。
DOI：
10.1167/iovs.15-18365
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发表时间：
2016-01-01
影响因子：
4.4
通讯作者：
Shukla D
中科院分区：
文献类型：
--
作者：
Jaishankar D;Buhrman JS;Valyi-Nagy T;Gemeinhart RA;Shukla D
延长硫酸肝素结合肽G2-C的释放，使用市售的隐形眼镜作为递送载体，并通过角膜HSV-1感染的体外、离体和体内模型证明释放的肽阻断单纯疱疹病毒1 （HSV-1）感染的能力。将市售隐形眼镜浸泡在肽溶液中5天，然后在不同的时间点测定肽的释放。用MTT法和细胞周期法测定释放样品的细胞毒性，用病毒侵入法和人角膜上皮细胞（HCE）法测定释放样品的功能活性。并对其在人、猪和小鼠体内角膜模型中抑制感染的能力进行了评估。肽G2-C通过隐形眼镜释放。释放3天后，该肽通过抑制HSV-1病毒在HCE细胞中的进入和传播显示出显著的活性。在角膜的离体和体内实验中也观察到明显的感染抑制。通过市售隐形眼镜延长释放抗hs肽可产生显著的抗hsv -1活性，为控制角膜疱疹提供了一种新的有效途径。
To prolong the release of a heparan sulfate binding peptide, G2-C, using a commercially available contact lens as a delivery vehicle and to demonstrate the ability of the released peptide to block herpes simplex virus-1 (HSV-1) infection using in vitro, ex vivo, and in vivo models of corneal HSV-1 infection. Commercially available contact lenses were immersed in peptide solution for 5 days prior to determining the release of the peptide at various time points. Cytotoxicity of the released samples was determined by MTT and cell cycle analysis, and the functional activity of the released samples were assessed by viral entry, and viral spread assay using human corneal epithelial cells (HCE). The ability to suppress infection in human and pig cornea ex vivo and mouse in vivo models were also assessed. Peptide G2-C was released through the contact lens. Following release for 3 days, the peptide showed significant activity by inhibiting HSV-1 viral entry and spread in HCE cells. Significant suppression of infection was also observed in the ex vivo and in vivo experiments involving corneas. Extended release of an anti–HS peptide through a commercially available contact lens can generate significant anti–HSV-1 activity and provides a new and effective way to control corneal herpes.