Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis.
Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis.
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水稻和拟南芥胚芽样蛋白家族的全基因组表征和表达分析
DOI：
10.3390/ijms17101622
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发表时间：
2016-09-23
影响因子：
5.6
通讯作者：
Shen Z
中科院分区：
文献类型：
--
作者：
Li L;Xu X;Chen C;Shen Z
先前的研究表明，类似胚性蛋白(GLP)在水稻和拟南芥中普遍存在。然而，对它们在发育和非生物/生物胁迫抗性中的作用的了解仍然有限。在本研究中，我们报道了水稻和拟南芥中GLP基因家族的全基因组鉴定、特性、亚细胞定位、酶活性和表达分析，以研究它们的功能。通过系统分析，在水稻和拟南芥基因组中分别鉴定出43个和32个GLP。系统发育分析将GLP基因分为6个分支，在GLP基因的启动子中发现了许多与胁迫和发育相关的顺式元件。此外，亚细胞定位和超氧化物歧化酶(SOD)分析表明，随机选择的水稻8号和4号染色体上的OsGLP基因在具有SOD活性的细胞壁中表达。总体而言，我们的结果表明，串联复制事件，特别是水稻8号染色体上的串联复制基因簇，在GLP家族的扩大中发挥了重要作用，从而加深了我们对GLP家族在非生物/生物胁迫和发育中的作用的理解。
Previous studies have shown that germin-like proteins (GLPs) are present ubiquitously in rice and Arabidopsis. However, the understanding regarding their role in development and abiotic/biotic stress resistance remains limited. In the present study, we report genome-wide identification, characterisation, subcellular localization, enzyme activity, and expression analysis of the GLP gene family in rice and Arabidopsis to study their functions. In total, 43 and 32 GLPs in the rice and Arabidopsis genome were identified based on a systematic analysis, respectively. The GLP genes were clustered into six clades based on phylogenetic analysis, and many stress and developmental-related cis-elements were detected in promoters of GLP genes. In addition, subcellular location and superoxide dismutase (SOD) analysis demonstrated that the random selected OsGLP genes on chromosomes 8 and 4 of rice were expressed in the cell wall with SOD activity. Overall, our results showed that tandem duplication events, especially the clusters of tandem duplication genes on chromosome 8 in rice, play a major role in expansion of the GLP family and thus increase our understanding of the role of the GLP family in abiotic/biotic stress and development.
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影响因子：
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作者：
Dubey S;Misra P;Dwivedi S;Chatterjee S;Bag SK;Mantri S;Asif MH;Rai A;Kumar S;Shri M;Tripathi P;Tripathi RD;Trivedi PK;Chakrabarty D;Tuli R
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DOI：
10.1016/j.bbrc.2014.05.027
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影响因子：
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DOI：
10.1016/j.bbrc.2010.10.073
发表时间：
2010-11-26
影响因子：
3.1
作者：
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