Xeno Nucleic Acid Nanosensors for Enhanced Stability Against Ion-Induced Perturbations
Xeno Nucleic Acid Nanosensors for Enhanced Stability Against Ion-Induced Perturbations
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DOI：
10.1021/acs.jpclett.8b01879
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发表时间：
2018-08-02
影响因子：
5.7
通讯作者：
Boghossian, Ardemis A.
中科院分区：
文献类型：
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作者：
Gillen, Alice J.;Kupis-Rozmyslowicz, Justyna;Boghossian, Ardemis A.
生物流体中无处不在的盐类给设计适合活体应用的传感器带来了无处不在的挑战。离子浓度的波动已被证明影响基于单链DNA包裹的单壁碳纳米管(ssDNA-SWCNTs)光学传感器的灵敏度和选择性。在此，我们观察到在浓度高于3.5 mM的二价阳离子存在下，基于单链DNA-SWCNT的光学传感器的荧光波长发生了移动。相比之下，使用异种核酸(XNAs)进行生物工程的传感器在浓度高达350 mM时没有观察到漂移。瞬时荧光测量显示，在离子诱导的构象变化过程中，基于单链DNA和XNA的包裹发生了明显的光学转变，基于XNA的传感器显示出构象和信号稳定性的持久性增加。这项演示引入了合成生物学作为增强纳米管光电行为的补充手段，开启了开发具有增强能力的纳米生物工程传感器的先前未探索的可能性。
The omnipresence of salts in biofluids creates a pervasive challenge in designing sensors suitable for in vivo applications. Fluctuations in ion concentrations have been shown to affect the sensitivity and selectivity of optical sensors based on single-walled carbon nanotubes wrapped with single-stranded DNA (ssDNA-SWCNTs). We herein observe fluorescence wavelength shifting for ssDNA-SWCNT-based optical sensors in the presence of divalent cations at concentrations above 3.5 mM. In contrast, no shifting was observed for concentrations up to 350 mM for sensors bioengineered with increased rigidity using xeno nucleic acids (XNAs). Transient fluorescence measurements reveal distinct optical transitions for ssDNA- and XNA-based wrappings during ion-induced conformation changes, with XNA-based sensors showing increased permanence in conformational and signal stability. This demonstration introduces synthetic biology as a complementary means for enhancing nanotube optoelectronic behavior, unlocking previously unexplored possibilities for developing nanobioengineered sensors with augmented capabilities.