Non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors
Non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors
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DOI：
10.1038/s41598-018-38363-z
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发表时间：
2019-02-07
影响因子：
4.6
通讯作者：
Finnemann, Silvia C.
中科院分区：
文献类型：
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作者：
Mazzoni, Francesca;Muller, Claudia;Finnemann, Silvia C.
磷脂酰丝氨酸外化是细胞凋亡的早期分子标志。在许多视网膜变性疾病中，感光细胞神经元通过凋亡而死亡。在这里，我们报告了双（锌（II）-吡啶二甲基胺（Zn-DPA）与德克萨斯红（PSVue-550）的磷脂酰丝氨酸结合缀合物在活体色素或白化病大鼠和视网膜变性小鼠中瞬时标记凋亡光感受器的效用。将PSVue-550用作眼药水是无毒的，无需眼内注射。PSVue-550荧光特异性且短暂地标记垂死的视网膜光感受器，使用标准视网膜或整个小动物成像系统可以在麻醉动物中检测到。重要的是，之前的PSVue-550滴眼液给药和成像不会影响重复检测。总之，我们的研究结果建立了PSVue-550成像作为一种完全非侵入性的方法，提供了在临床前研究中纵向监测视网膜感光细胞死亡的机会。
Phosphatidylserine externalization is an early molecular signature for apoptosis. In many retinal degenerative diseases, photoreceptor neurons die by apoptosis. Here, we report utility of the phosphatidylserine-binding conjugate of Bis(zinc(II)-dipicolylamine (Zn-DPA) with Texas-red (PSVue-550) in transiently labeling apoptotic photoreceptors in living pigmented or albino rats and mice with retinal degeneration. Applying PSVue-550 as eyedrop is non-toxic and eliminates need for intraocular injection. PSVue-550 fluorescence specifically and transiently labeling dying retinal photoreceptors is detectable in anesthetized animals using standard retinal or whole small animal imaging systems. Importantly, prior PSVue-550 eyedrop administration and imaging does not affect repeat testing. Altogether, our results establish PSVue-550 imaging as a completely non-invasive method that provides the opportunity to longitudinally monitor retinal photoreceptor cell death in preclinical studies.