Development and Characterization of a Genotype 4 Hepatitis E Virus Cell Culture System Using a HE-JF5/15F Strain Recovered from a Fulminant Hepatitis Patient
Development and Characterization of a Genotype 4 Hepatitis E Virus Cell Culture System Using a HE-JF5/15F Strain Recovered from a Fulminant Hepatitis Patient
复制标题
DOI：
10.1128/jcm.00629-09
复制
发表时间：
2009-06-01
影响因子：
9.4
通讯作者：
Okamoto, Hiroaki
中科院分区：
文献类型：
--
作者：
Tanaka, Toshinori;Takahashi, Masaharu;Okamoto, Hiroaki
我们利用从一名重型肝炎患者中分离到的HE-JF5/15F株建立了一种高效的4型戊型肝炎病毒的细胞培养系统。培养上清液中的第六代病毒在接种后第10天达到1.5×108拷贝/毫升，有10个核苷酸突变和4个氨基酸变化。
We developed an efficient cell culture system for genotype 4 hepatitis E virus using the HE-JF5/15F strain recovered from a fulminant hepatitis patient. The sixth-passage virus in the culture supernatant reached 1.5 x 108 copies/ml at 10 days postinoculation and possessed 10 nucleotide mutations with four amino acid changes.