Relationship of germinal centers in lymphoid tissue to immunologic memory. VI. Transfer of B cell memory with lymph node cells fractionated according to their receptors for peanut agglutinin.
Relationship of germinal centers in lymphoid tissue to immunologic memory. VI. Transfer of B cell memory with lymph node cells fractionated according to their receptors for peanut agglutinin.
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DOI：
10.4049/jimmunol.131.5.2254
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发表时间：
1983-11
影响因子：
4.4
通讯作者：
R. Coico;B. Bhogal;G. Thorbecke
中科院分区：
文献类型：
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作者：
R. Coico;B. Bhogal;G. Thorbecke
我们利用生发中心 B 细胞对花生凝集素 (PNA) 的高亲和力来分离它们。通过在 PNA 包被的培养皿上淘选分离出 PNA+ 和 PNA- B 细胞，检查它们在启动后不同时间将记忆反应转移到受辐射受体的能力。使用在生发中心形成高峰时从淋巴结中分离的 B 细胞，在 PNA+ B 细胞群的受体中获得了最大的反应。在引发后 4 至 5 周以及用无关抗原攻击后 10 天，PNA+ 或 PNA- B 细胞的接受者的记忆反应大致相同。启动后 14 周，仅在 PNA-B 细胞群的接受者中发现记忆反应。脾脏中的记忆 B 细胞取自 8 周前在足垫中进行免疫处理的小鼠，也是 PNA- 的。最后，我们表明，在同一足垫中启动 6 个月后，在足垫中使用 TNP 结合物进行增强，诱导引流节点的 PNA+ B 细胞部分重新出现显着的记忆反应性。
We isolated germinal center B cells by exploiting their high affinity for peanut agglutinin (PNA). The PNA+ and PNA- B cells, fractionated by panning on PNA-coated petri dishes, were examined for their ability to transfer memory responses to irradiated recipients at various times after priming. With such fractionated B cells from lymph nodes taken at the peak of germinal center formation, the largest response was obtained in recipients of the PNA+ B cell population. At 4 to 5 wk after priming, and 10 days after challenge with an unrelated antigen, memory responses were approximately equal in recipients of PNA+ or PNA- B cells. At 14 wk after priming, memory responses were found only in recipients of the PNA- B cell population. Memory B cells from the spleen, taken from mice primed in the footpad 8 wk earlier, were also PNA-. Finally, we show that boosting with a TNP-conjugate in the footpad, 6 mo after priming in the same footpad, induced the reappearance of marked memory responsiveness in the PNA+ B cell fraction of the draining node.