PHYSICAL MAPPING OF THE MITOCHONDRIAL GENOME OF ARABIDOPSIS-THALIANA BY COSMID AND YAC CLONES
PHYSICAL MAPPING OF THE MITOCHONDRIAL GENOME OF ARABIDOPSIS-THALIANA BY COSMID AND YAC CLONES
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DOI：
10.1046/j.1365-313x.1994.06030447.x
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发表时间：
1994-09-01
期刊：
影响因子：
7.2
通讯作者：
SCHUSTER, W
中科院分区：
文献类型：
--
作者：
KLEIN, M;ECKERTOSSENKOPP, U;SCHUSTER, W
作为世界范围内对拟南芥作为模式植物进行分子分析的一部分，线粒体基因组的完整结构已经确定。通过对300多个cosmid克隆和纯化的线粒体DNA进行限制性内切片段分析，绘制了线粒体DNA分子图谱。372 kb的整个基因组包含在三种不同结构的圆形分子中，并分别被分成两个额外的234 kb和138 kb的亚基因组分子。这些排列是由反向和/或正向组合中存在的两组重复序列的重组引起的。YAC克隆的比对证实了拟南螺旋体线粒体中存在超过300kb的连续DNA分子。这个相对较大的线粒体基因组存在于一个具有最小核基因组之一的植物中，表明不同的大小约束作用于植物细胞中不同的基因组。
As part of the worldwide efforts at molecular analysis of Arabidopsis thaliana as a model plant the complete structure of the mitochondrial genome has been determined. The mitochondrial DNA molecules were mapped by restriction fragment analysis of more than 300 cosmid clones and purified mitochondrial DNA. The entire genome of 372 kb is contained in three different configurations of circular molecules and is split into two additional subgenomic molecules of 234 kb and 138 kb, respectively. These arrangements result from recombinations of the two sets of repeats present in combinations of inverted and/or direct orientation. Alignment of YAC clones confirms the in vivo presence of continuous DNA molecules of more than 300 kb in A. thaliana mitochondria. The presence of this comparatively large mitochondrial genome in a plant with one of the smallest nuclear genomes shows that different size constraints act upon the different genomes in plant cells.