Bacterial exopolysaccharides: Chemical structures, gene clusters and genetic engineering
Bacterial exopolysaccharides: Chemical structures, gene clusters and genetic engineering
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细菌胞外多糖：化学结构、基因簇和基因工程
DOI：
10.1016/j.ijbiomac.2021.01.139
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发表时间：
2021
影响因子：
8.2
通讯作者：
Zhang Jianfa
中科院分区：
文献类型：
--
作者：
Sun Xiaqing;Zhang Jianfa
近几十年来，人们对细菌胞外多糖（EPS）的组成、结构、生物合成和功能进行了广泛的研究。EPS通过不同的生物合成途径合成。负责EPS合成的基因通常聚集在细菌的基因组或大质粒上。一般来说，不同的EPS合成基因簇指导合成具有不同化学结构和生物活性的EPS。更好地了解EPS生物合成中涉及的基因功能对于生产具有特殊生物活性的EPS至关重要。EPS合成相关基因的研究通常采用基因工程的方法。本文对EPS的结构、相关基因的合成等方面的文献进行了梳理，重点介绍了利用基因编辑方法修饰EPS基因簇的研究进展。
In recent decades, the composition, structure, biosynthesis, and function of bacterial extracellular polysaccharides (EPS) have been extensively studied. EPS are synthesized through different biosynthetic pathways. The genes responsible for EPS synthesis are usually clustered on the genome or large plasmids of bacteria. Generally, different EPS synthesis gene clusters direct the synthesis of EPS with different chemical structures and biological activities. A better understanding of the gene functions involved in EPS biosynthesis is critical for the production of EPS with special biological activities. Genetic engineering methods are usually used to study EPS synthesis related genes. This review organizes the available information on EPS, including their structures, synthesis of related genes, and highlights the research progress of modifying EPS gene clusters through gene-editing methods.