Expression Profile of the REG Gene Family in Colorectal Carcinoma
Expression Profile of the REG Gene Family in Colorectal Carcinoma
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DOI：
10.1369/jhc.2010.956961
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发表时间：
2011-01-01
影响因子：
3.2
通讯作者：
Takano, Yasuo
中科院分区：
文献类型：
--
作者：
Zheng, Hua-chuan;Sugawara, Akira;Takano, Yasuo
再生（REG）基因家族属于钙依赖性凝集素基因超家族，编码小的多功能分泌蛋白，可能参与细胞增殖、分化和癌变。为了阐明REG在结直肠癌（CRC）中的表达谱，作者通过组织芯片免疫组化检测了REG I α、I β、III、HIP/PAP和REG IV的表达。REG I α、III和HIP/PAP的表达在crc中比邻近非肿瘤性粘膜更常见（p < 0.001），而在REG I β和IV中则相反（p < 0.001）。REG I α、I β、III和HIP/PAP的表达与crc侵袭深度呈负相关（p < 0.05）。REG I β和HIP/ PAP在有静脉浸润的crc中的表达低于无静脉浸润的crc （p < 0.05）。REG I α和HIP/ PAP阳性率在未发生淋巴结转移的crc中显著高于未发生淋巴结转移的crc （p < 0.05）。黏液癌表达REG IV蛋白的频率高于分化良好、中分化的黏液癌（p < 0.05）。REG I α、I β、III与HIP/ PAP表达呈正相关（p < 0.05）。生存分析显示REG I β或HIP/ PAP表达与肿瘤患者预后呈正相关（p < 0.05）。本研究提示，异常的REG表达可能与crc的发病、侵袭或淋巴结转移密切相关。REG I β和HIP/ PAP可被认为是CRC患者预后良好的可靠标志。（中国生物医学工程学报，2011）
Regenerating (REG) gene family belongs to the calcium-dependent lectin gene superfamily and encodes small multifunctional secretory proteins, which might be involved in cell proliferation, differentiation, and carcinogenesis. To clarify REG expression profile in colorectal carcinoma (CRC), the authors examined the expression of REG I alpha, I beta, III, HIP/PAP, and REG IV by immunohistochemistry on tissue microarray. The expression of REG I alpha, III, and HIP/PAP was more frequently observed in the CRCs than adjacent non-neoplastic mucosa (p < 0.001), whereas it was the converse for REG I beta and IV (p < 0.001). The expression of REG I alpha, I beta, III, and HIP/PAP was negatively correlated with the depth of invasion of CRCs (p < 0.05). The REG I beta and HIP/ PAP were less expressed in CRCs with than without venous invasion (p < 0.05). The positive rates of REG I alpha and HIP/ PAP were significantly higher in CRCs without than with lymph node metastasis (p < 0.05). Mucinous carcinoma more frequently expressed REG IV protein than well-and moderately differentiated ones (p < 0.05). There was a positive relationship between REG I alpha, I beta, III, and HIP/ PAP expression (p < 0.05). Survival analysis indicated the REG I beta or HIP/ PAP expression was positively linked to favorable prognosis of carcinoma patients (p < 0.05). This study indicated that aberrant REG expression might be closely linked to the pathogenesis, invasion, or lymph node metastasis of CRCs. REG I beta and HIP/ PAP could be considered reliable markers of favorable prognosis of CRC patients. (J Histochem Cytochem 59:106-115, 2011)