Rapid increase in enzyme and peptide mRNA in sympathetic ganglia after electrical stimulation in humans.
Rapid increase in enzyme and peptide mRNA in sympathetic ganglia after electrical stimulation in humans.
复制标题
人体电刺激后交感神经节中酶和肽 mRNA 迅速增加。
DOI：
10.1073/pnas.86.11.4302
复制
发表时间：
1989
影响因子：
11.1
通讯作者：
Hökfelt,T
中科院分区：
文献类型：
--
作者：
Schalling,M;Stieg,PE;Lindquist,C;Goldstein,M;Hökfelt,T
用合成儿茶酚胺合成酶酪氨酸羟基酶(EC 1.14.16.2)和多巴胺β-羟基酶(EC 1.14.17.1)和神经肽酪氨酸的寡核苷酸探针原位杂交研究电刺激节前刺激后的人胸神经节。并进行免疫组织化学分析。围手术期短时间刺激后，主神经节细胞中三种基因的表达均增加数倍，而免疫组织化学检测不到明显的酶或多肽水平的变化。这些结果表明，在人类的交感神经系统中，参与信号传输的基因受到了非常迅速和敏感的调节。此外，它们表明，电刺激神经元和/或通路结合原位杂交可以作为一种方法，通过可视化靶细胞中递质酶和/或肽的mRNAs的增加来确定神经元投射。
Thoracic ganglia in humans were studied after electrical, preganglionic stimulation using in situ hybridization with synthetic oligonucleotide probes against the catecholamine-synthesizing enzymes tyrosine hydroxylase (EC 1.14.16.2) and dopamine beta-hydroxylase (EC 1.14.17.1) and neuropeptide tyrosine. Immunohistochemical analysis was also performed. Following short peroperative stimulation a severalfold increase in all three mRNAs was found in principal ganglion cells, whereas no definite changes could be detected in enzyme or peptide levels with immunohistochemistry. The results suggest a very rapid and sensitive regulation of genes involved in signal transmission in the sympathetic nervous system of humans. Moreover, they indicate that electrical stimulation of neurons and/or pathways combined with in situ hybridization may be used as a method to define neuronal projections by visualizing increases in mRNAs for transmitter enzymes and/or peptide in target cells.