CHANGES IN TOTAL RNA, POLYADENYLATED RNA, AND ACTIN MESSENGER-RNA DURING MEIOTIC MATURATION OF MOUSE OOCYTES
CHANGES IN TOTAL RNA, POLYADENYLATED RNA, AND ACTIN MESSENGER-RNA DURING MEIOTIC MATURATION OF MOUSE OOCYTES
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DOI：
10.1016/0012-1606(85)90036-3
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
PAYNTON, BV
中科院分区：
文献类型：
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作者：
BACHVAROVA, R;DELEON, V;PAYNTON, BV
用溴化乙锭荧光测定小鼠卵母细胞的总RNA含量，发现在减数分裂成熟过程中减少19%（排卵卵的RNA含量比完全发育的卵母细胞少19%）。成熟卵母细胞的预标记稳定RNA在体外成熟过程中减少了约20%。在完全发育的卵母细胞中，聚腺苷化RNA占总预标记RNA的19%，在体外成熟的卵母细胞中占10%，证实了之前在体内制备材料上的结果。为了区分1 mRNA的死蛋白化和降解，我们使用带有镍翻译的。β探针的卵母细胞RNA的Northern印迹检测了肌动蛋白mRNA的腺苷化的数量和状态。-肌动蛋白克隆鸡cDNA。成熟过程中肌动蛋白mRNA的数量保持不变，但其分子量略有下降。用寡聚物（dT）和RNase H处理RNA的实验表明，肌动蛋白mRNA在成熟过程中死基化，此时肌动蛋白合成已知会下降。在胚胎发生的前1 1/2天内，先前定义的大量RNA丢失和mRNA腺苷化状态的变化实际上开始于受精前减数分裂成熟的12小时。
The total RNA content of mouse oocytes, as measured by ethidium bromide fluorescence, was found to decrease by 19% during meiotic maturation (ovulated eggs contain 19% less RNA than full-grown oocytes). Prelabeled stable RNA of full-grown oocytes decreased by about 20% during in vitro maturation. Polyadenylated RNA represented 19% of total prelabeled RNHA in full-grown oocytes and 10% in oocytes matured in vitro, confirming previous results on in vivo prepared material. To distinguish between deadenylation and degradation for 1 mRNA, the amount and state of adenylation of actin mRNA was examined using Northern blots of oocyte RNA probed with a nick-translated .beta.-actin cloned chicken cDNA. The amount of actin mRNA remained similar during maturation, but its MW decreased slightly. Experiments in which RNA was treated with oligo(dT) and RNase H demonstrated that the actin mRNA was deadenylated during maturation, when actin synthesis was known to decline. The previously defined loss of bulk RNA and changes in the state of adenylation of mRNA during the first 1 1/2 days of embryogenesis actually begin during the 12 h of meiotic maturation preceding fertilization.