CFL1, a WW Domain Protein, Regulates Cuticle Development by Modulating the Function of HDG1, a Class IV Homeodomain Transcription Factor, in Rice and Arabidopsis
CFL1, a WW Domain Protein, Regulates Cuticle Development by Modulating the Function of HDG1, a Class IV Homeodomain Transcription Factor, in Rice and Arabidopsis
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DOI：
10.1105/tpc.111.088625
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发表时间：
2011-09-01
期刊：
影响因子：
11.6
通讯作者：
Gu, Hongya
中科院分区：
文献类型：
--
作者：
Wu, Renhong;Li, Shibai;Gu, Hongya
植物有一个化学成分不均匀的亲脂层，角质层，保护它们免受生物和非生物胁迫。调节角质层发育的机制知之甚少。我们鉴定了一个水稻（Oryza sativa）显性卷曲叶突变体，卷曲旗叶1（cfl 1），并克隆了编码WW结构域蛋白的CFL 1。我们在拟南芥中过表达水稻和拟南芥CFL 1;这些转基因植物表现出严重受损的角质层发育，类似于CFL 1水稻。减少表达的CFL 1导致角质层结构的加强。在CFL 1主要表达在专门的表皮细胞和开裂和脱落发生的区域。生化证据表明，在CFL 1与HDG 1，IV类同源结构域亮氨酸拉链转录因子相互作用。HDG 1功能的抑制导致类似的缺陷角质层表型在野生型拟南芥，但大大减轻表型在Cfl 1 -1突变体。两个角质层发育相关基因BDG和FDH的表达在AtCFL 1过表达和HDG 1抑制植物中下调。HDG 1与BDG和FDH的调控区中存在的顺式元件L1盒结合。我们的研究结果表明，水稻和拟南芥CFL 1通过影响HDG 1的功能来负调控角质层发育，HDG 1调节下游基因BDG和FDH。
Plants have a chemically heterogeneous lipophilic layer, the cuticle, which protects them from biotic and abiotic stresses. The mechanisms that regulate cuticle development are poorly understood. We identified a rice (Oryza sativa) dominant curly leaf mutant, curly flag leaf1 (cfl1), and cloned CFL1, which encodes a WW domain protein. We overexpressed both rice and Arabidopsis CFL1 in Arabidopsis thaliana; these transgenic plants showed severely impaired cuticle development, similar to that in cfl1 rice. Reduced expression of At CFL1 resulted in reinforcement of cuticle structure. At CFL1 was predominantly expressed in specialized epidermal cells and in regions where dehiscence and abscission occur. Biochemical evidence showed that At CFL1 interacts with HDG1, a class IV homeodomain-leucine zipper transcription factor. Suppression of HDG1 function resulted in similar defective cuticle phenotypes in wild-type Arabidopsis but much alleviated phenotypes in At cfl1-1 mutants. The expression of two cuticle development-associated genes, BDG and FDH, was downregulated in At CFL1 overexpressor and HDG1 suppression plants. HDG1 binds to the cis-element L1 box, which exists in the regulatory regions of BDG and FDH. Our results suggest that rice and Arabidopsis CFL1 negatively regulate cuticle development by affecting the function of HDG1, which regulates the downstream genes BDG and FDH.