The CLN3/SWI6/CLN2 pathway and SNF1 act sequentially to regulate meiotic initiation in Saccharomyces cerevisiae
The CLN3/SWI6/CLN2 pathway and SNF1 act sequentially to regulate meiotic initiation in Saccharomyces cerevisiae
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DOI：
10.1046/j.1365-2443.2002.00551.x
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发表时间：
2002-07-01
期刊：
影响因子：
2.1
通讯作者：
Honigberg, SM
中科院分区：
文献类型：
--
作者：
Purnapatre, K;Piccirillo, S;Honigberg, SM
背景资料：IME 1是减数分裂启动所必需的，受Cln 3：Cdc 28激酶和Snf 1激酶的调节，Cln 3：Cdc 28激酶激活G1-to-S转换，Snf 1激酶介导葡萄糖阻遏。在这里，我们研究的途径，Cln 3：Cdc 28 p抑制IME 1和Cln 3：Cdc 28 p和Snf 1 p之间的关系，在这种regulation.Results：当野生型酵母停止生长，他们表达IME 1到中等水平，中间的低水平之间的增长过程中表达的高水平，在孢子形成。即使在生长过程中，在cln 3 Delta、cln 1 Delta、cln 2 Delta、cdc 28 -4和swi 6 Delta突变体中也发生中度IME 1表达。这些突变体也比野生型更快地诱导IME 1表达。CLN 3需要SWI 6和CLN 2来抑制IME 1和IME 2，但CLN 1在这种抑制中的活性远低于CLN 2。cln 3 Delta snf 1 Delta双突变体的表型表明Cln 3：Cdc 28 p独立于SNF 1调节IME 1。结论：进入减数分裂涉及两个独立但顺序的控制，它们通过三位开关调节IME 1：（i）在生长过程中，IME 1被CLN 3/SWI 6/CLN 2途径抑制，（ii）一旦生长停止，这种抑制被释放，IME 1以中等水平表达，和（iii）随后，激活Snf 1 p的营养条件允许高IME 1表达。
Background: IME1 , which is required for the initiation of meiosis, is regulated by Cln3:Cdc28 kinase, which activates the G1-to-S transition, and Snf1 kinase, which mediates glucose repression. Here we examine the pathway by which Cln3:Cdc28p represses IME1 and the relationship between Cln3:Cdc28p and Snf1p in this regulation.Results: When wild-type yeast cease growth, they express IME1 to moderate levels, intermediate between the low levels expressed during growth and the high levels expressed during sporulation. Moderate IME1 expression occurred in cln3Delta , cln1Delta cln2Delta , cdc28-4 and swi6Delta mutants, even during growth. These mutants also induced IME1 expression more rapidly than the wild-type. CLN3 required SWI6 and CLN2 to repress IME1 and IME2 , but CLN1 was much less active than CLN2 in this repression. The phenotype of the cln3Delta snf1Delta double mutant indicated that Cln3:Cdc28p regulates IME1 independently of SNF1 .Conclusion: Entry into meiosis involves two independent but sequential controls, which regulate IME1 via a three position switch: (i) during growth IME1 is repressed by the CLN3/SWI6/CLN2 pathway, (ii) once growth ceases, this repression is released and IME1 is expressed at moderate levels, and (iii) subsequently, nutritional conditions that activate Snf1p allow high IME1 expression.