Optical Transmittance for Analysis of the Dynamics of Self-Assembled Rotating Chains of Superparamagnetic Micro- and Nano Beads in Solution
Optical Transmittance for Analysis of the Dynamics of Self-Assembled Rotating Chains of Superparamagnetic Micro- and Nano Beads in Solution
复制标题
光学透射率用于分析溶液中超顺磁微纳米珠自组装旋转链的动力学
DOI：
10.1088/1742-6596/352/1/012002
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Pil Ju Ko
中科院分区：
文献类型：
--
作者：
T.Fukushima;K.-W.Lee;T.Tanaka;M.Koyanagi;M. Natsui;Pil Ju Ko
光学监测生物传感程序的基础上的动态的水溶液中含有功能化的超顺磁珠的应用程序的外部旋转磁场已经开发了一个快速，高灵敏度，和廉价的生物测定。通常，微米直径珠粒的动力学通过常规光学显微镜观察。为了对生物分子具有更大的亲和力，需要使用纳米尺寸的超顺磁珠，其尺寸与实际生物分子相当。然而，由于显微镜的最大分辨率，到目前为止，用于监测纳米尺寸珠粒的动力学的工作量有限。在这里，我们提出了一种新的协议，使我们能够通过光透射率的变化监测纳米直径珠的动态。
Optically monitoring biosensing procedures based on the dynamics of an aqueous solution containing functionalized superparamagnetic beads in the application of the external rotating magnetic field has been developed for a rapid, highly sensitive, and inexpensive bioassay. Typically, the dynamics of micrometer diameter beads is observed by conventional optical microscopes. For greater affinity to biomolecules, there is a demand which necessitates the use of nanometer sized superparamagnetic beads, comparable size to actual biomolecules. However, a limited amount of work for monitoring the dynamics of nanometer sized beads has been performed thus far due to the maximum resolution of microscopes. Here, we propose a novel protocol enabling us monitor the dynamics of nanometer-diameter beads via change in the optical transmittance.