Recognition memory-induced gene expression in the perirhinal cortex: A transcriptomic analysis.
Recognition memory-induced gene expression in the perirhinal cortex: A transcriptomic analysis.
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DOI：
10.1016/j.bbr.2017.04.007
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发表时间：
2017-06-15
影响因子：
2.7
通讯作者：
Uney JB
中科院分区：
文献类型：
--
作者：
Scott H;Rogers MF;Scott HL;Campbell C;Warburton EC;Uney JB
转录组的特征是在接触到新的或熟悉的物体后。观察到转录因子和GDNF受体的差异表达。编码细胞外基质蛋白的基因有差异表达。检测到了选择性剪接的差异。我们已经使用转录组分析来识别在识别记忆形成过程中被激活的基因和通路。老鼠被反复接触物体，以便熟悉这些物体，或者在连续六天的蝴蝶结迷宫中接触新物体。在最后一天，也就是最后一次接触这一系列物体的一小时后，来自虹膜周围皮质的RNA被测序，以比较天真对照组大鼠和暴露在新的或熟悉的刺激下的大鼠的转录组。在新生组大鼠和熟悉组大鼠之间发现了差异表达基因。这些基因包括转录因子、GDNF受体和细胞外基质相关蛋白的编码基因。此外，两组在选择性剪接上的差异也被检测到，这表明这种转录后机制可能在物体识别记忆的巩固中发挥作用。总之，这项研究表明，RNA测序可以作为一种工具来识别经历相同任务但遇到不同暴露的行为动物的基因表达差异。
The transcriptome was characterised following exposure to novel or familiar objects. Differential expression of transcription factors and GDNF receptors was observed. Genes coding for extracellular matrix proteins are differentially expressed. Differences in alternative splicing were detected. We have used transcriptome analysis to identify genes and pathways that are activated during recognition memory formation in the perirhinal cortex. Rats were exposed to objects either repeatedly, so that the objects become familiar, or to novel objects in a bow-tie maze over six consecutive days. On the final day, one hour after the last exposure to the series of objects, RNA from the perirhinal cortex was sequenced to compare the transcriptome of naïve control rats and rats exposed to either novel or familiar stimuli. Differentially expressed genes were identified between group Novel and group Familiar rats. These included genes coding for transcription factors, GDNF receptors and extracellular matrix-related proteins. Moreover, differences in alternative splicing were also detected between the two groups, which suggests that this post-transcriptional mechanism may play a role in the consolidation of object recognition memory. To conclude, this study shows that RNA sequencing can be used as a tool to identify differences in gene expression in behaving animals undergoing the same task but encountering different exposures.