6-sulfo sialyl Lewis X is the common receptor determinant recognized by H5, H6, H7 and H9 influenza viruses of terrestrial poultry.
6-sulfo sialyl Lewis X is the common receptor determinant recognized by H5, H6, H7 and H9 influenza viruses of terrestrial poultry.
复制标题
6-Sulfo siallyl Lewis X是陆地家禽H5，H6，H7和H9流感病毒所识别的常见受体决定因素。
DOI：
10.1186/1743-422x-5-85
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发表时间：
2008-07-24
期刊：
影响因子：
4.8
通讯作者：
Klimov AI
中科院分区：
文献类型：
--
作者：
Gambaryan AS;Tuzikov AB;Pazynina GV;Desheva JA;Bovin NV;Matrosovich MN;Klimov AI
家禽的甲型流感病毒起源于水禽的自然宿主，是种间传播和适应新宿主物种的结果。我们先前注意到，从不同目的水禽和陆鸟中分离的流感病毒可能通过识别Neu5Acα2-3Gal末端唾液酸寡糖受体的内部结构而在其精细的受体结合特异性上有所不同。为了进一步表征这些差异，我们研究了来自野生水禽、家禽、猪和马的一大组甲型流感病毒的受体结合特性。使用竞争固相结合试验，我们确定了病毒与唾液酸寡糖聚合物的结合，这些聚合物结合的唾液酸寡糖与Neu5Acα-Gal连接的类型和寡糖链较远部分的结构不同。从陆生家禽中分离的流感病毒与鸭病毒的不同之处在于，它与含有唾液酸寡糖的硫酸和/或岩藻糖化的Neu5Acα2-3半乳糖的结合增强。大多数测试的禽类病毒与6-磺基唾液酸基Lewis X(SU-SLeX)有很高的结合亲和力。家禽病毒与SU-SLeX的有效结合往往伴随着它们与Neu5Acα2-6Gal末端(人型)受体的结合能力。这种双重受体结合的特异性在北美和欧亚H7病毒、H9N2欧亚禽类病毒以及从猪分离的H1、H3和H9禽类病毒中被证明。陆生家禽流感病毒与祖先鸭病毒的不同之处在于增强了与硫酸和/或岩藻糖化的Neu5Acα2-3Gal末端受体的结合，偶尔还与Neu5Acα2-6Gal末端(人型)受体结合。这些发现表明，对禽类受体的适应可以增强禽流感病毒在禽类与人之间传播和大流行传播的可能性。
Influenza A viruses of domestic birds originate from the natural reservoir in aquatic birds as a result of interspecies transmission and adaptation to new host species. We previously noticed that influenza viruses isolated from distinct orders of aquatic and terrestrial birds may differ in their fine receptor-binding specificity by recognizing the structure of the inner parts of Neu5Acα2-3Gal-terminated sialyloligosaccharide receptors. To further characterize these differences, we studied receptor-binding properties of a large panel of influenza A viruses from wild aquatic birds, poultry, pigs and horses. Using a competitive solid-phase binding assay, we determined viral binding to polymeric conjugates of sialyloligosaccharides differing by the type of Neu5Acα-Gal linkage and by the structure of the more distant parts of the oligosaccharide chain. Influenza viruses isolated from terrestrial poultry differed from duck viruses by an enhanced binding to sulfated and/or fucosylated Neu5Acα2-3Gal-containing sialyloligosaccharides. Most of the poultry viruses tested shared a high binding affinity for the 6-sulfo sialyl Lewis X (Su-SLex). Efficient binding of poultry viruses to Su-SLex was often accompanied by their ability to bind to Neu5Acα2-6Gal-terminated (human-type) receptors. Such a dual receptor-binding specificity was demonstrated for the North American and Eurasian H7 viruses, H9N2 Eurasian poultry viruses, and H1, H3 and H9 avian-like virus isolates from pigs. Influenza viruses of terrestrial poultry differ from ancestral duck viruses by enhanced binding to sulfated and/or fucosylated Neu5Acα2-3Gal-terminated receptors and, occasionally, by the ability to bind to Neu5Acα2-6Gal-terminated (human-type) receptors. These findings suggest that the adaptation to receptors in poultry can enhance the potential of an avian virus for avian-to-human transmission and pandemic spread.