Quantification of metabolic flux in plant secondary metabolism by a biogenetic organizational approach.
Quantification of metabolic flux in plant secondary metabolism by a biogenetic organizational approach.
复制标题
通过生物遗传组织方法量化植物次生代谢中的代谢通量。
DOI：
10.1006/mben.2002.0224
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发表时间：
2002
影响因子：
8.4
通讯作者：
Shanks,JacquelineV
中科院分区：
文献类型：
--
作者：
Morgan,JohnA;Shanks,JacquelineV
代谢工程是一种很有前途的方法，可以提高植物中有价值的天然产物的产量。一种通过代谢物测量来量化通量的方法对于分析天然途径和修饰途径是必要的。而不是只关注最终产物的积累，分析广泛的次级代谢物具有显著的优势。我们提出了一个模型，通过分组相似生物合成来源的代谢物来组织通量分析。为此，我们量化了长春花毛状根培养中吲哚生物碱途径的几个分支的代谢物的时间谱。通过分析这些数据，我们能够检查关键分支点周围的通量分布。此外，该分析还提供了重要的信息，如对次级代谢总通量的估计。
Metabolic engineering represents a promising approach to enhance the yield of valuable natural products from plants. A method to quantify flux through metabolite measurements is necessary for the analysis of native and modified pathways. Rather than focusing only on the accumulation of the final products, analyzing a wide range of secondary metabolites has significant advantages. We propose a model that organizes the flux analysis by grouping metabolites of similar biosynthetic origin. To this end, we have quantified temporal profiles of metabolites from several branches of the indole alkaloid pathway in Catharanthus roseus hairy root cultures. By analyzing these data, we are able to examine the distribution of flux around key branchpoints. Furthermore, this analysis provides crucial information such as an estimate of total flux to secondary metabolism.