Phytate degradation by human gut isolated Bifidobacterium pseudocatenulatum ATCC27919 and its probiotic potential
Phytate degradation by human gut isolated Bifidobacterium pseudocatenulatum ATCC27919 and its probiotic potential
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DOI：
10.1016/j.ijfoodmicro.2009.07.015
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发表时间：
2009-09-30
影响因子：
5.4
通讯作者：
Andlid, Thomas
中科院分区：
文献类型：
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作者：
Haros, Monika;Carlsson, Nils-Gunnar;Andlid, Thomas
人们日益认识到饮食与健康之间的关系，这导致了除了提供基本营养外，对支持健康的食品的需求也在增加。益生菌是存在于食品中的活生物体，它与胃肠道的相互作用产生与健康相关的好处。植酸酶是磷酸酶的一个亚类，催化植酸盐的脱磷，从而减少其对矿物生物有效性的负面影响，并产生较低的肌醇磷酸盐。本研究的目的是(I)研究益生菌候选双歧杆菌在合成介质中降解植酸的能力，(Ii)鉴定产生的较低的肌醇磷酸盐，(Iii)研究其在模拟胃肠道条件下的存活情况，以及(Iv)评估细菌与Caco-2细胞的粘附性。拟卡氏杆菌植酸降解酶/S降解InSP(6)的第一步优先在肌醇醇环的DL-6和5-位启动。结果表明，拟卡氏假单胞菌降解InsP6的主要途径是D/L-INS(1，2，3，4，5)P-5或D/L-INS(1，2，3，4，6)P-5，D/L-INS(1，2，3，4)P-4，最后是INS(1，2，3)P-3和D/L-INS(1，2，4)P-3/D/L-INS(1，3，4)P-3。这种人类菌株对胆汁也表现出显著的耐受性，对人类肠道Caco-2细胞的选择性黏附能力(对对照表面的黏附为零)，与商业益生菌B.lactis相当。植酸降解活性构成了一种新的代谢特性，作为一种对人体肠道具有潜在营养作用的营养益生菌特性，它可能有助于改善肠道中的矿物质吸收。(C)2009爱思唯尔B.V.保留所有权利。
The growing awareness of the relationship between diet and health has led to an increasing demand for food products that support health above and beyond providing basic nutrition. Probiotics are live organisms present in foods, which yield health benefits related to their interactions with the gastrointestinal tract. Phytases are a subgroup of phosphatases that catalyse the desphosphorylation of phytate, which reduces its negative impact on mineral bioavailability, and generates lower inositol phosphates. The aims of this investigation were to (i) study the ability of the probiotic candidate Bifidobacterium pseudocatenulatum to degrade phytate in synthetic medium, to (ii) identify the lower inositol phosphates generated, to (iii) study its survival under conditions mimicking gastrointestinal passage and finally to (iv) assess adhesion of the bacteria to Caco-2 cells. The first steps of InsP(6) degradation by B. pseudocatenulatum phytate-degrading enzyme/s were preferentially initiated at the DL-6-position and 5-position of the myo-inositol ring. It suggests that the main InsP6 degradation pathway by B. pseudocatenulatum by sequential removal of phosphate groups was D/L-Ins(1,2,3,4,5)P-5 or D/L-Ins(1,2,3,4,6)P-5; D/L-Ins(1,2,3,4)P-4; to finally Ins(1,2,3)P-3 and D/L-Ins(1,2,4)P-3/D/L-Ins(1,3,4)P-3. This human strain also showed a notable tolerance to bile as well as a selective adhesion capacity (adhesion to control surfaces was zero), to human intestinal Caco-2 cells comparable to the commercial probiotic B. lactis. The phytate-degrading activity constitutes a novel metabolic trait which could contribute to the improvement of mineral absorption in the intestine as a nutritional probiotic feature with potential trophic effect ill human gut. (C) 2009 Elsevier B.V. All rights reserved.