A label-free phosphorescence sensing platform for trypsin based on Mn-ZnS QDs
A label-free phosphorescence sensing platform for trypsin based on Mn-ZnS QDs
复制标题
基于Mn-ZnS量子点的胰蛋白酶无标记磷光传感平台
DOI：
10.1039/c7ra03349d
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Dong, Chuan
中科院分区：
文献类型：
--
作者：
Liu, Wei;Li, Huanhuan;Dong, Chuan
采用 Mn 掺杂 ZnS 量子点 (Mn-ZnS QD) 作为磷光探针，开发了一种用于胰蛋白酶的无标记磷光传感平台。 Mn-ZnS量子点可以发出强的室温磷光。添加细胞色素 c (Cyt c) 后，Mn-ZnS QD 和 Cyt c 之间形成杂化复合物，从而猝灭 Mn-ZnS QD 的磷光。在胰蛋白酶存在下，Cyt c 被水解成小片段，因此，Mn-ZnS QD 的猝灭磷光恢复并观察到高磷光强度。因此，基于系统的磷光信号变化，构建了无标记的开启磷光传感平台。综合探讨了Mn-ZnS量子点浓度、反应时间、温度和pH等影响胰蛋白酶测定的因素。在最佳实验条件下，传感系统恢复的磷光对胰蛋白酶的响应范围为 0.88–15.6 μg mL−1，检测限为 42 ng mL−1。此外，该传感平台还成功应用于人血清样品中胰蛋白酶的测定，回收率令人满意。
A label free phosphorescence sensing platform for trypsin was developed employing Mn doped ZnS quantum dots (Mn-ZnS QDs) as the phosphorescence probe. Mn-ZnS QDs can emit strong room temperature phosphorescence. Upon addition of Cytochrome c (Cyt c), a hybrid complex between Mn-ZnS QDs and Cyt c was formed, and consequently, quenched the phosphorescence of Mn-ZnS QDs. In the presence of trypsin, Cyt c was hydrolyzed into small fragments, and accordingly, the quenched phosphorescence of Mn-ZnS QDs went back and high phosphorescence intensity was observed. As such, based on the phosphorescence signal changes of the system, a label free turn-on phosphorescence sensing platform was fabricated. Factors affecting the determination of trypsin including the concentration of Mn-ZnS QDs, reaction time, temperature and pH effect were comprehensively explored. Under the optimal experimental conditions the restored phosphorescence of the sensing system displayed a response for trypsin in the range of 0.88–15.6 μg mL−1 with a detection limit of 42 ng mL−1. Additionally, the sensing platform was successfully applied for the determination of trypsin in human serum samples with satisfactory recovery.