Effects of 1,5-anhydro-D-glucitol on insulin secretion both in in vitro and ex vivo pancreatic preparations
Effects of 1,5-anhydro-D-glucitol on insulin secretion both in in vitro and ex vivo pancreatic preparations
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1,5-脱水-D-葡萄糖醇对体外和离体胰腺制剂中胰岛素分泌的影响
DOI：
10.1016/j.jphs.2022.03.005
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发表时间：
2022
影响因子：
3.5
通讯作者：
Fujita Tomoe
中科院分区：
文献类型：
--
作者：
Ouchi Motoshi;Morita Asuka;Satoh Keitaro;Kobayashi Shunsuke;Terada Misao;Kon Hiroe;Hayashi Keitaro;Suzuki Tatsuya;Oba Kenzo;Sugihara Hitoshi;Yasutake Masahiro;Anzai Naohiko;Fujita Tomoe
器官浴实验通常用于研究激素和药物对器官反应的生理作用和影响。我们建立了一种从离体大鼠胰腺制剂中复制胰岛素分泌的实验方法，以研究促进胰岛素分泌的物质。1,5-无水- d -葡萄糖醇（1,5- ag）存在于食物中，存在于人类和啮齿动物中；然而，1,5- ag是否刺激胰岛素分泌尚不清楚。本研究旨在评估短期1,5- ag刺激对体外和大鼠源性INS-1E细胞胰岛素分泌的影响。结果表明，1,5- ag在体内和体外实验中均没有增加胰岛素流出比例的效力。体外用10 μM格列美脲（磺脲类药物）刺激后胰岛素流出量显著增加。葡萄糖刺激的胰岛素分泌不仅在INS-1E细胞中，而且在大鼠胰腺制剂中也被观察到。我们的研究结果表明，短期暴露于1,5- ag对大鼠的胰岛素分泌没有影响。
Organ bath experiments are conventionally used to investigate the physiological actions and effects of hormones and drugs on organ responses. We developed an experimental method to reproduce insulin secretion from isolated rat pancreas preparations, to investigate substances that promote insulin secretionex vivo. 1,5-anhydro-D-glucitol (1,5-AG) is found in foods, and exists in humans and rodents; however, whether 1,5-AG stimulates insulin secretion remains unclear. This study aimed to assess the effects of short-term 1,5-AG stimulation on insulin secretion in bothex vivoand in INS-1E (rat-derived) cellsin vitro. Our results indicated that 1,5-AG had no potency to increase the proportion of insulin outflow both inex vivoandin vitroexperiments. Insulin outflow significantly increased upon stimulation with 10 μM glimepiride, a member of the sulfonylurea class of drugs,ex vivo. Glucose-stimulated insulin secretion was observed not only in INS-1E cells but also in rat pancreatic preparations. Our findings demonstrated that short-term exposure to 1,5-AG had no effect on insulin secretion in rats.