Spectral and thermodynamic properties of methanobactin from γ-proteobacterial methane oxidizing bacteria: A case for copper competition on a molecular level
Spectral and thermodynamic properties of methanobactin from γ-proteobacterial methane oxidizing bacteria: A case for copper competition on a molecular level
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DOI：
10.1016/j.jinorgbio.2010.08.002
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发表时间：
2010-12-01
影响因子：
3.9
通讯作者：
DiSpirito, Alan A.
中科院分区：
文献类型：
--
作者：
Choi, Dong W.;Bandow, Nathan L.;DiSpirito, Alan A.
甲烷双子素（MB）是一种低分子质量铜结合分子，类似于铁结合铁载体。该分子是由许多甲烷营养或甲烷氧化细菌（BOB）产生的，但仅在一个暴民中才表征在一个暴民中，即Trichosporium ob3b。为了探索这一新型金属结合化合物中的潜在分子多样性，光谱（可见的，荧光和电子顺磁共振共鸣）和来自两个Gamma-蛋白细菌MOB的MB的热力学特性。确定并比较来自α-杆菌虫M. trichosporium ob3b的MB，并比较甲基甲状腺囊肿和甲基菌粒专辑BG8。来自两个γ-细菌病的MB与MB在分子质量和光谱特性中的MB不同于MB。与来自Trichosporium ob3b的MB相比，细胞外浓度较低，来自两个γ-细菌BOB的MB的铜结合常数也很低。此外，来自M. trichosporium ob3b的MB从两个γ-细菌群的MB中除去了Cu（i）。综上所述，结果表明MB可能是调节铜限制环境中甲烷营养社区结构的一个因素。 （c）2010 Elsevier Inc.保留所有权利。
Methanobactin (mb) is a low molecular mass copper-binding molecule analogous to iron-binding siderophores. The molecule is produced by many methanotrophic or methane oxidizing bacteria (MOB), but has only been characterized to date in one MOB, Methylosinus trichosporium OB3b. To explore the potential molecular diversity in this novel class of metal binding compound, the spectral (UV-visible, fluorescent, and electron paramagnetic resonance) and thermodynamic properties of mb from two gamma-proteobacterial MOB. Methylococcus capsulatus Bath and Methylomicrobium album BG8, were determined and compared to the mb from the alpha-proteobacterial MOB, M. trichosporium OB3b. The mb from both gamma-proteobacterial MOB differed from the mb from M. trichosporium O83b in molecular mass and spectral properties. Compared to mb from M. trichosporium OB3b, the extracellular concentrations were low, as were copper-binding constants of mb from both gamma-proteobacterial MOB. In addition, the mb from M. trichosporium OB3b removed Cu(I) from the mb of both gamma-proteobacterial MOB. Taken together the results suggest mb may be a factor in regulating methanotrophic community structure in copper-limited environments. (C) 2010 Elsevier Inc. All rights reserved.