Adjuvant-active fraction from Albizia julibrissin saponins improves immune responses by inducing cytokine and chemokine at the site of injection
Adjuvant-active fraction from Albizia julibrissin saponins improves immune responses by inducing cytokine and chemokine at the site of injection
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合欢皂苷的佐剂活性成分通过在注射部位诱导细胞因子和趋化因子来改善免疫反应
DOI：
10.1016/j.intimp.2014.07.021
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发表时间：
2014-10-01
影响因子：
5.6
通讯作者：
Shi, Minghua
中科院分区：
文献类型：
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作者：
Sun, Hongxiang;He, Shuwang;Shi, Minghua
合欢皮总皂苷（AJS（t））在小鼠体内具有良好的佐剂作用。本研究将AJS（t）进行树脂柱层析，得到AJS（30）、AJS（50）、AJS（75）和AJS（95）四个组分，并进一步比较了这四个组分对鸡卵清蛋白（OVA）和重组禽痘病毒载体禽流感疫苗（rFPV）的溶血活性和免疫佐剂潜力。AJS（t）、AJS（99）、AJS（75）和AJS（95）显示轻微溶血作用。AJS（t）、AJS（50）和AJS（75）不仅能显著促进ConA、LPS和抗原刺激的小鼠脾细胞增殖，而且能显著提高OVA和rFPV免疫小鼠血清中抗原特异性IgG、IgG 1、IgG 2a和IgG 2b抗体滴度。AJS（t）、AJS（50）和AJS（75）还能显著促进OVA免疫小鼠的NK细胞活性和迟发型超敏反应（DTH）。此外，通过使用抗体阵列确定AJS（75）对注射部位的细胞因子和趋化因子的影响来探索佐剂作用的机制。AJS 75诱导或上调注射肌肉中12种细胞因子（IL-12 p40、IL-12 p40/p70、IFN-γ、IL-13、IL-1 β、IL-6、IL-10、TNF-α、sTNFR I、sTNFR III、IL-3和IL-9）和10种趋化因子（Eotaxin、I-TAC、TNF、MIP-1 α、RANTES、TECK、Fracatlkine、Fast、M-CSF和GM-CSF）的蛋白表达。结果表明，AJS（75）是AJS（t）中最具抗炎活性的组分，它能增强抗原特异性的细胞和体液免疫应答，并通过诱导注射部位细胞因子和趋化因子的产生，同时诱导Th 1/Th 2应答。(C)2014爱思唯尔有限公司版权所有。
The total saponin from the stem bark of Albizia julibrissin (AJS(t)) has previously showed the adjuvant potentials in mice. In this study, AJS(t) was subjected to resin column chromatography to afford four fractions (AJS(30), AJS(50), AJS(75) and AJS(95)), and these fractions were further compared for the hemolytic activities and adjuvant potentials on the immune response to ovalbumin (OVA) and recombinant fowl pox virus vector-based avian influenza vaccine (rFPV). AJS(t), AJS(99), AJS(75) and AJS(95) showed a slight hemolytic effect. AJS(t), AJS(50) and AJS(75) significantly enhanced not only the concanavalin A (Con A)-, lipopolysaccharide (LPS)- and antigen-stimulated splenocyte proliferation, but also serum antigen-specific IgG, IgG1, IgG2a and IgG2b antibody titers in the mice immunized with OVA and rFPV. AJS(t), AJS(50) and AJS(75) also significantly promoted the NK cell activity and delayed-type hypersensitivity (DTH) in the OVA-immunized mice. Furthermore, the mechanisms of adjuvant action were explored by determining the effects of AJS(75) on cytokines and chemokines at the site of injection using antibody array. AJS75 induced or up-regulated the protein expression of 12 cytokines (IL-12p40, IL-12p40/p70, IFN-gamma, IL-13, IL-1 beta, IL-6, IL-10, TNF-alpha, sTNFR I, sTNFR III, IL-3 and IL-9) and 10 chemokines (Eotaxin, I-TAC, MIG, MIP-1 alpha, RANTES, TECK, Fracatlkine, Fast, M-CSF and GM-CSF) in the injected muscles. The results suggested that AJS(75), the most adjuvant-active fraction of AJS(t), could improve antigen-specific both cellular and humoral immune responses and simultaneously elicit a Th1/Th2 response by inducing cytokine and chemokine at the site of injection. (C) 2014 Elsevier B.V. All rights reserved.