Autographa californica nuclear polyhedrosis virus: Subcellular localization and protein trafficking of BV/ODV-E26 to intranuclear membranes and viral envelopes
Autographa californica nuclear polyhedrosis virus: Subcellular localization and protein trafficking of BV/ODV-E26 to intranuclear membranes and viral envelopes
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DOI：
10.1006/viro.1997.8903
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发表时间：
1998-01-05
期刊：
影响因子：
3.7
通讯作者：
Summers, MD
中科院分区：
文献类型：
--
作者：
Beniya, H;Braunagel, SC;Summers, MD
加州签名虫核型多角体病毒da26基因编码出芽病毒（BV）和闭塞衍生病毒（ODV）的包膜蛋白。感染后4小时（p.i.）， Western blot和时间分析检测到26 kDa的蛋白。蛋白质含量以每小时16小时的速度增加，并在整个感染过程中保持较高水平。在36 h p.i.时，检测到一些额外的免疫反应蛋白，它们在大约18 kDa的位置迁移并保持到96 h p.i.。纯化的病毒包膜和核衣壳制备的Western blot分析表明，26和18 kDa的蛋白都是BV和ODV包膜的结构蛋白。在仅存在26 kda蛋白的情况下进行免疫电子显微镜检查，证实该蛋白位于ODV包膜上。该蛋白被命名为BV/ODV-E26，以表示与病毒子代的结合、包膜位置和表观分子量。追踪BV/ODV- e26定位的研究表明，在感染早期，该蛋白被整合到细胞质囊泡中，并在16小时后，在与病毒诱导的核内微囊泡和ODV包膜相关的细胞核中检测到BV/ODV- e26。共免疫沉淀和酵母双杂交实验表明BV/ODV-E26和FP25K能够相互作用形成复合物，共免疫沉淀实验表明细胞肌动蛋白是该复合物的第三组分。总之，这些数据表明，FP25K和细胞肌动蛋白可能参与杆状病毒蛋白和/或病毒核衣壳在细胞内的调控或运动。(C) 1998学术出版社。
The Autographa californica nuclear polyhedrosis virus da26 gene codes for an envelope protein of both budded virus (BV) and occlusion derived virus (ODV). Western blot and temporal analysis of infected cell extracts detected a protein of 26 kDa by 4 h postinfection (p.i.). The amount of protein increased by 16 h p.i. and remained at high levels throughout infection. By 36 h p.i. several additional immunoreactive proteins were detected which migrated at approximate to 18 kDa and remained through 96 h p.i. Western blot analysis of purified virus envelope and nucleocapsid preparations revealed that both the 26- and 18-kDa proteins are structural proteins of the envelope of BV and ODV. Immunoelectron microscopy performed at a time when only the 26-kDa species of the protein was present confirmed that the protein located to ODV envelope. The protein was named BV/ODV-E26 to designate incorporation into viral progeny, envelope location, and apparent molecular weight. Studies designed to follow localization of BV/ODV-E26 demonstrated that early in infection, the protein was incorporated into cytoplasmic vesicles and by 16 h p.i., BV/ODV-E26 was detected in the nucleus associated with virus-induced intranuclear microvesicles and ODV envelope. Coimmunoprecipitation and yeast two-hybrid assays showed that BV/ODV-E26 and FP25K were capable of interacting with each other to form a complex and coimmunoprecipitation assays indicated that cellular actin was a third component of this complex. Together, these data suggest that FP25K and cellular actin may participate in the regulation, or movement through the cell, of baculovirus proteins and/or Virus nucleocapsids. (C) 1998 Academic Press.