Immunodetection of collagen types I, II, III, and IV for differentiation of liver fibrosis stages in patients with chronic HCV
Immunodetection of collagen types I, II, III, and IV for differentiation of liver fibrosis stages in patients with chronic HCV
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DOI：
10.1080/15321810701212088
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Shaker, Yehia M.
中科院分区：
文献类型：
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作者：
Attallah, Abdelfattah M.;Mosa, Tamer E.;Shaker, Yehia M.
本研究旨在评估血清胶原和其他血清生化指标作为慢性丙型肝炎病毒（HCV）相关性肝纤维化的有用的非侵入性标志物。使用ELISA和Western印迹技术检测血清中的I、II、III和IV型胶原。随着纤维化分期的进展，胶原I、II、III和IV的ELISA水平显著增加。基于受试者工作特征（ROC）曲线分析，III型胶原（70 kDa）和IV型胶原（200 kDa）比其他血清生物标志物更有助于区分重度纤维化和轻度纤维化。多变量判别分析（MDA）选择纤维化判别评分（FDS）= [ 2.345 +胶原III（μ g/mL）x 1.923 +胶原IV（μ g/mL）x 1.544 + ALT（U/mL）x 0.005] - [白蛋白（g/L）x 0.046]。FDS在临界评分= 2.2时正确分类了87%的严重纤维化患者（即，大于2.2表示重度肝纤维化，小于2.2表示轻度肝纤维化），特异性为97%。在一项验证研究中，将FDS应用于第二组患者，结果重现，无显著差异。总之，开发的基于四参数的FDS可用于识别慢性HCV感染患者的严重肝纤维化。
The current study is aimed at evaluating serum collagens and other serum biochemical markers as useful, non-invasive markers of hepatic fibrosis associated with chronic hepatitis C virus ( HCV). Collagen types I, II, III, and IV were detected in serum using ELISA and Western blot techniques. The ELISA levels of collagen I, II, III, and IV increased significantly with the progression of fibrosis staging. Based on receiver-operating characteristic ( ROC) curve analysis, the collagen type III ( 70 kDa) and type IV ( 200 kDa) were more useful than other serum bio-markers for differentiating severe fibrosis from mild fibrosis. Multivariate discriminant analysis ( MDA) selected a fibrosis discriminant score ( FDS) = [ 2.345 + Collagen III ( mu g/mL) x 1.923 + Collagen IV ( mu g/mL) x 1.544 + ALT ( U/mL) x 0.005] - [ albumin( g/L) x 0.046]. The FDS correctly classified 87% of the severe fibrosis patients at a cut-off score = 2.2 ( i.e., more than 2.2 indicated severe fibrotic liver and less than 2.2 indicated mild fibrotic liver) with specificity of 97%. In a validation study, the FDS was applied to the second cohort of patients and the results were reproduced without significant difference. In conclusion, the developed four-parameter based FDS is useful for identifying severe liver fibrosis in patients with chronic HCV infection.