Compressive strains at physiological frequencies influence the metabolism of chondrocytes seeded in agarose
Compressive strains at physiological frequencies influence the metabolism of chondrocytes seeded in agarose
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DOI：
10.1002/jor.1100150205
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发表时间：
1997-03-01
影响因子：
2.8
通讯作者：
Bader, DL
中科院分区：
文献类型：
--
作者：
Lee, DA;Bader, DL
关节软骨受到动态压缩负荷，这是已知的影响软骨细胞代谢。虽然确切的信号机制尚不清楚，但已经提出细胞变形发挥作用，并可能通过不同的途径刺激代谢反应。本研究利用琼脂糖包埋软骨细胞的模型系统，研究了动态细胞应变对三个关键代谢过程的影响，即糖胺聚糖合成、DNA合成和总蛋白合成。使用专门设计的装置，在一定的频率范围内(0.3-3赫兹)将15%的压缩应变幅度静态或动态地施加到琼脂糖软骨细胞柱体上。静态和低频应变(0.3赫兹)抑制糖胺多糖的合成，而1赫兹的频率刺激合成。静态应变降低了胸腺嘧啶核苷的摄取水平，而所有频率的动态应变都诱导了软骨细胞增殖的增加。所有受试菌株方案都抑制了氚-脯氨酸的掺入。所研究的三个参数都以不同的方式受到动态应变方案的影响，这意味着涉及的信号机制是不耦合的。
Articular cartilage is subjected to dynamic compressive loading that is known to influence chondrocyte metabolism. While the exact signalling mechanisms are unclear, it has been proposed that cell deformation plays a role and may stimulate a metabolic response through distinctive pathways. In this study, a well characterized model system in which chondrocytes are embedded in agarose was used to study the effect of dynamic cellular strain on three key metabolic processes, namely the synthesis of glycosaminoglycan, of DNA, and of total protein. Using a specially designed apparatus, 15% compressive strain amplitude was applied to agarose-chondrocyte cylinders statically or dynamically over a range of frequencies (0.3-3 Hz). Static and low-frequency strain (0.3 Hz) inhibited the synthesis of glycosaminoglycan, while a frequency of 1 Hz stimulated synthesis. Static strain reduced the level of thymidine uptake, whereas dynamic strain at all frequencies induced an increase in chondrocyte proliferation. Incorporation of tritiated proline was suppressed by all strain regimens tested. The three parameters investigated were each influenced by the dynamic strain regimens in a distinct manner, implying that the signalling mechanisms involved are uncoupled.