[Effect of moxibustion on TLR4/MyD88/NF-kappaB signaling pathway in colon of diarrhea-predo-minant irritable bowel syndrome rats].
[Effect of moxibustion on TLR4/MyD88/NF-kappaB signaling pathway in colon of diarrhea-predo-minant irritable bowel syndrome rats].
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DOI：
10.13702/j.1000-0607.190950
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发表时间：
2020-08-25
期刊：
影响因子：
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通讯作者：
Cheng, Hong-Liang
中科院分区：
文献类型：
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作者：
Chu, Hao-Ran;Wang, Yu;Cheng, Hong-Liang
目的：观察艾灸对腹泻型肠易激综合征（IBS-D）大鼠血清炎症因子、toll样受体4 （TLR4）、髓样分化因子88 （MyD88）及结肠组织核因子κ B （nf - κ B）的影响，探讨艾灸治疗IBS-D的作用机制。方法：将健康雄性SD大鼠随机分为空白组、模型组和艾灸组，每组8只。采用慢性抑制联合灌胃番泻叶液建立IBS-D模型。艾灸组大鼠在“天枢”（ST25）和“上聚虚”（ST37）灸30 min，每天1次，连用7 d。干预后观察稀便率和结直肠膨胀引起的腹停反射（AWR）最小阈值体积。采用ELISA法检测血清炎症因子il -1 β、tnf - α和IL-6的含量。采用H.E.染色法观察大鼠结肠的病理变化。免疫组织化学检测结肠组织中TLR4和NF-kappaB （p65）的平均光密度。采用实时荧光定量PCR检测结肠组织中TLR4、MyD88和NF-kappaB (p65) mrna的相对表达量，Western blot检测结肠组织中TLR4、MyD88和NF-kappaB （p65）蛋白的表达量。结果：模型组大鼠结肠有轻度炎症反应。与空白组比较，模型组大鼠AWR最小体积阈值显著降低（P< 0.01），稀便率、血清中il -1 β、IL-6和tnf - α含量以及结肠组织中TLR4、MyD88、NF-kappaB (p65) mrna和蛋白的表达均显著升高（P< 0.01）
OBJECTIVE: To observe the effect of moxibustion on serum inflammatory factors, Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88) and nuclear factor Kappa B (NF-kappaB) in colon tissue of rats with diarrhea-predominant irritable bowel syndrome (IBS-D)，so as to explore the mechanism of moxibustion in the treatment of IBS-D.METHODS: Healthy male SD rats were randomly divided into blank group, model group and moxibustion group, with 8 rats in each group. The IBS-D model was established by chronic restraint combined with gavage of Senna-leaf solution. The rats of the moxibustion group was treated with moxibustion at "Tianshu"(ST25)and "Shangjuxu"(ST37)for 30 min， once a day for 7 d. After the intervention, the rate of loose stools and the minimum threshold volume of abdominal withdrawal reflex (AWR) induced by colorectal distension were observed. The contents of serum inflammatory factors IL-1beta, TNF-alpha and IL-6 were detected by ELISA. The pathological changes of rat colon were observed by H.E. staining. The average optical density of TLR4 and NF-kappaB (p65) in colon tissue was detected by immunohistochemistry. The relative expressions of TLR4, MyD88 and NF-kappaB (p65) mRNAs in colon tissues were detected by real-time fluorescence quantitative PCR, and the expression levels of TLR4, MyD88 and NF-kappaB (p65) proteins in colon tissues were detected by Western blot.RESULTS: There was a mild degree of inflammation in colon in rats of the model group. Compared with the blank group, the minimum volume threshold of AWR decreased significantly (P< 0.01), and the rate of loose stools, the contents of IL-1beta, IL-6 and TNF-alpha in serum as well as the expressions of TLR4, MyD88, NF-kappaB (p65) mRNAs and protein in colon tissues were significantly increased in the model group (P