NEW REST PERIOD PROTOCOL FOR INDUCING TOLERANCE TO HIGH O-2 EXPOSURE IN ADULT-RATS
NEW REST PERIOD PROTOCOL FOR INDUCING TOLERANCE TO HIGH O-2 EXPOSURE IN ADULT-RATS
复制标题
DOI：
10.1152/ajplung.1989.257.4.l226
复制
发表时间：
1989-10-01
影响因子：
--
通讯作者：
MASSARO, D
中科院分区：
文献类型：
--
作者：
FRANK, L;IQBAL, J;MASSARO, D
我们报告了一种新方案，用于诱导成年大鼠对长期暴露于高氧环境的显着耐受性，该方案需要在暴露于通常致死浓度的氧气之间使用单一的“休息期”。成年大鼠暴露于 > 95% O2 中 48 小时，然后在空气中休息，或者甚至在 50-75% O2 中休息，始终导致 100% 存活，有证据表明，在继续暴露于 > 95% O2 3-7 天期间，仅出现轻微肺水肿（大鼠在室内空气中休息 24 小时，7 天存活率 = 23/23；对于在 50-75% O2 中休息的大鼠，7 天存活率O2 24 小时 = 27/27；连续暴露于 O2 的对照大鼠 = 0/11）。高氧耐受性的诱导与休息后再次暴露于 > 95% O2 期间肺部抗氧化酶活性的显着增加相关。该协议中的事件导致肺抗氧化酶活性增加的分子手段仅部分已知，但由于产生了显着的耐受性，因此阐明该机制对于我们理解高氧耐受性至关重要。
We report a new protocol for inducing marked tolerance to prolonged exposure to hyperoxia in adult rats that entails the use of a single "rest period" between exposures to a usually lethal concentration of O2. Exposure of adult rats to > 95% O2 for 48 h followed by a rest in air, or a rest even in 50-75% O2, consistently resulted in 100% survival with evidence of only slight pulmonary edema during continuation of exposure to > 95% O2 for 3-7 more days (7-day survival for rats rested in room air for 24 h = 23/23; for rats rested in 50-75% O2 for 24 h = 27/27; for continuously O2- exposed control rats = 0/11). Induction of tolerance to hyperoxia was associated with significant increases in the lungs'' antioxidant enzyme activities during the reexposure to > 95% O2 following the rest period. The molecular means by which the events in this protocol lead to increased lung antioxidant enzyme activity is only partially known, but because of the marked tolerance produced, the elucidation of the mechanisms must be important to our understanding of tolerance to hyperoxia.