Perovskite Nanocrystal Fluorescence-Linked Immunosorbent Assay Methodology for Sensitive Point-of-Care Biological Test
Perovskite Nanocrystal Fluorescence-Linked Immunosorbent Assay Methodology for Sensitive Point-of-Care Biological Test
复制标题
用于敏感床旁生物测试的钙钛矿纳米晶体荧光联免疫吸附测定方法
DOI：
10.1016/j.matt.2020.05.004
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发表时间：
2020-07-01
期刊：
影响因子：
18.9
通讯作者：
Zeng, Haibo
中科院分区：
文献类型：
--
作者：
Dong, Yuhui;Tang, Xiaoqian;Zeng, Haibo
定量荧光免疫分析法是食品安全和临床卫生保健现场检测的一种重要而便捷的方法。然而，目前的标记材料，从有机荧光素到量子点，由于其发射范围太广，仍然缺乏对多个感兴趣目标的需求。本文报道了一种具有更窄光致发光光谱的新型标记材料——钙钛矿纳米晶体（Pe-NCs），并初步论证了一种灵敏、定量的免疫分析方法——钙钛矿纳米晶体荧光联免疫吸附法。通过羟基官能化，解决了实现水分散稳定的Pe-NCs的关键问题。荧光探针（钙钛矿抗体）采用静电吸附法构建，半最大全宽较窄（近似于18 nm），为今后多靶点检测的研究奠定基础。间接竞争免疫和夹心免疫分析均获得成功。这项工作为POC检测提供了一种可能的标记材料，并可能为下一代免疫测定开辟一条有前途的途径。
Quantitative fluorescence immunoassay is a vital and convenient method in point-of-care (POC) testing for food safety and clinical healthcare. However, current marking materials, from organic luciferin to quantum dots, still lack the requirement of increasing need for multiple targets of interest because of their too broad emission. Here, a new label material, perovskite nanocrystals (Pe-NCs), with a narrower photoluminescence spectrum is reported, and a sensitive and quantitative immunoassay method named the perovskite nanocrystal fluorescence-linked immunosorbent assay is preliminarily demonstrated. The key point of realizing water-dispersed and stable Pe-NCs was addressed by functionalizing with hydroxyl groups. The fluorescent probes (perovskite antibody) were constructed via electrostatic adsorption with narrow full-width at half-maximum (similar to 18 nm), laying the foundation for future study of multi-target detection. Both indirect competitive immunity and sandwich immunoassay were successfully demonstrated. This work provides a possible label material for POC tests and may open up a promising avenue of next-generation immunoassay.