Scintigraphic detection of regional disruption of adrenergic neurons in the heart.
Scintigraphic detection of regional disruption of adrenergic neurons in the heart.
复制标题
闪烁扫描检测心脏中肾上腺素能神经元的区域破坏。
DOI：
10.1016/0002-8703(88)90251-7
复制
发表时间：
1988
影响因子：
4.8
通讯作者：
Wieland,DM
中科院分区：
文献类型：
--
作者：
Sisson,JC;Lynch,JJ;Johnson,J;JaquesJr,S;Wu,D;Bolgos,G;Lucchesi,BR;Wieland,DM
实验旨在检测活体狗心脏中肾上腺素能神经元的区域性破坏。通过将苯酚应用于左心室心外膜来实现神经元破坏。去神经支配的证据是内源性去甲肾上腺素（NE）浓度的降低，在苯酚治疗区域下方的心肌，并向顶点。放射性标记的间碘苄胍（MIBG）作为NE的类似物，集中在肾上腺素能神经末梢。应用酚后20小时，发现125 I标记的MIBG在心肌内的分布模式与NE相似。然而，左胸骨切除术并没有改变NE或125 I-MIBG在心肌中的分布，显然没有破坏肾上腺素能神经支配。用123 I标记的MIBG使注射后3小时和20小时的活犬心脏神经元的放射性成像成为可能;这些图像描绘了由苯酚处理引起的肾上腺素能神经元破坏的区域。放射性核素图像上显示的铊-201浓度和体外染色观察到的三苯基四氮唑浓度均未显示心肌损伤。因此，用123 I-MIBG的动脉造影将显示心脏中的局部肾上腺素能去神经。
Experiments were designed to detect regional disruptions of adrenergic neurons in the hearts of living dogs. The neuron disruption was achieved by the application of phenol to the epicardium of the left ventricle. Evidence for denervation was the reduction in endogenous norepinephrine (NE) concentrations in the myocardium beneath the region of phenol treatment and toward the apex. Radiolabeledmeta-iodobenzylguanidine (MIBG) acts as an analog of NE and as such is concentrated in adrenergic nerve terminals. Following phenol application, MIBG labeled with125I was found, 20 hours after injection, to be distributed within myocardium in patterns comparable to those of NE. However, left stellectomy did not alter the distributions of NE or125I-MIBG in the myocardium and apparently did not disrupt adrenergic innervation. MIBG labeled with123I enabled scintigraphic images of heart neurons in the living dog 3 and 20 hours after injection; these images portrayed the regions of adrenergic neuron disruption caused by phenol treatment. Concentrations of thallium-201 depicted on scintigraphic image and of triphenyltetrazolium observed on in vitro staining demonstrated no myocardial injury. Thus scintigraphy with123I-MIBG will display regional adrenergic denervations in the heart.