NEW APPLICATION OF HIGH-PRESSURE REVERSED-PHASE LIQUID-CHROMATOGRAPHY IN LIPIDS
NEW APPLICATION OF HIGH-PRESSURE REVERSED-PHASE LIQUID-CHROMATOGRAPHY IN LIPIDS
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DOI：
10.1007/bf02534153
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发表时间：
1975-01-01
期刊：
影响因子：
1.9
通讯作者：
RAMACHANDRAN, S
中科院分区：
文献类型：
--
作者：
PEI, PTS;HENLY, RS;RAMACHANDRAN, S
高压反相液相色谱已用于分离饱和脂肪酸、其甲酯、多不饱和脂肪酸和甘油三酯。已经实现了链长和双键数量不同的脂肪酸的快速分离。大多数情况下分析时间少于 10 分钟。与传统的银硅酸柱色谱法相比，高压反相色谱法可以实现更好的多烯酸分离。分析是在化学键合反相填料 VYDAC 反相上进行的。
High pressure reverse‐phase liquid chromatography has been used to separate saturated fatty acids, their methyl esters, polyunsaturated fatty acids, and triglycerides. Rapid separation of fatty acids differing in chain length and number of double bonds has been accomplished. Analysis time was less than 10 min in most cases. The high pressure reverse chromatography resulted in better separations of polyenoic acids than can be accomplished by conventional argentation silicic acid column chromatography. The analyses were carried out on a chemically bonded reverse phase packing, VYDAC reverse phase.