Selective Suppression of Cell Growth and Programmed Cell Death-Ligand 1 Expression in HT1080 Fibrosarcoma Cells by Low Molecular Weight Fucoidan Extract
Selective Suppression of Cell Growth and Programmed Cell Death-Ligand 1 Expression in HT1080 Fibrosarcoma Cells by Low Molecular Weight Fucoidan Extract
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DOI：
10.3390/md17070421
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发表时间：
2019-07-01
期刊：
影响因子：
5.4
通讯作者：
Shirahata, Sanetaka
中科院分区：
文献类型：
--
作者：
Teruya, Kiichiro;Kusumoto, Yoshihiro;Shirahata, Sanetaka
低分子量岩藻聚糖硫酸酯提取物（LMF）是从新枝管藻中提取的岩藻聚糖硫酸酯粗品，经鲍鱼糖苷酶酶消化后得到的，具有多种生物活性，包括抗癌作用。各种癌症表达程序性细胞死亡配体1（PD-L1），已知其在逃避宿主免疫监视系统中起重要作用。PD-L1也在许多类型的正常细胞中表达，用于自我保护。先前的研究表明，选择性抑制癌细胞中表达的PD-L1对于成功根除癌症至关重要。在本研究中，我们分析了LMF是否可以调节HT 1080纤维肉瘤细胞中PD-L1的表达。我们的结果表明，LMF抑制PD-L1/PD-L2表达和HT 1080癌细胞的生长，而对正常TIG-1细胞的生长没有影响。因此，LMF差异调节正常细胞和癌细胞中的PD-L1表达，并且可以作为治疗具有高PD-L1表达的癌症的替代补充剂。
Low molecular weight fucoidan extract (LMF), prepared by an abalone glycosidase digestion of a crude fucoidan extracted from Cladosiphon novae-caledoniae Kylin, exhibits various biological activities, including anticancer effect. Various cancers express programmed cell death-ligand 1 (PD-L1), which is known to play a significant role in evasion of the host immune surveillance system. PD-L1 is also expressed in many types of normal cells for self-protection. Previous research has revealed that selective inhibition of PD-L1 expressed in cancer cells is critical for successful cancer eradication. In the present study, we analyzed whether LMF could regulate PD-L1 expression in HT1080 fibrosarcoma cells. Our results demonstrated that LMF suppressed PD-L1/PD-L2 expression and the growth of HT1080 cancer cells and had no effect on the growth of normal TIG-1 cells. Thus, LMF differentially regulates PD-L1 expression in normal and cancer cells and could serve as an alternative complementary agent for treatment of cancers with high PD-L1 expression.