Potential high virulence for infective endocarditis in Streptococcus mutans strains with collagen-binding proteins but lacking PA expression
Potential high virulence for infective endocarditis in Streptococcus mutans strains with collagen-binding proteins but lacking PA expression
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DOI：
10.1016/j.archoralbio.2013.06.008
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发表时间：
2013-11-01
影响因子：
3
通讯作者：
Nakano, K.
中科院分区：
文献类型：
--
作者：
Nomura, R.;Naka, S.;Nakano, K.
目的：变形链球菌是龋病的病原体，也是感染性心内膜炎（IE）的病原体。我们研究了表达胶原结合蛋白（CBP）的菌株，并根据190-kDa蛋白抗原（PA）的表达进行进一步分类。菌株TW 871的Zeta电位值（CBP+/PA+）和MT 8148（CBP-/PA+）和它们各自的PA缺陷型突变株TW 871 PD（CBP+/PA-）和MT 8148 PD（CBP-/PA-）的细胞粘附和侵袭人脐静脉内皮细胞（HUVECs）。从200名健康人的口腔菌株的分布进行了分析CBP和/或PA的表达和菌株的特点是他们的粘附和侵袭property.Results：TW 871 PD和MT 8148 PD表现出显着较低的zeta电位值比TW 871和MT 8148，分别。TW 871 PD的胶原结合率显著高于TW 871，但MT 8148和MT 8148 PD的胶原结合率几乎可以忽略不计。TW 871 PD组HUVECs的粘附率和侵袭率显著高于TW 871组，TW 871组显著高于MT 8148和MT 8148 PD组。CBP+菌株的患病率与10%相似，CBP+/PA-菌株的患病率与3%相似。对200株临床菌株的分析显示CBP+/PA-组的粘附率和侵袭率高于其他组。尽管变异株的分布频率较低，但它们对感染性心内膜炎的毒性可能很高。(C)2013爱思唯尔有限公司保留所有权利。
Objective: Streptococcus mutans, an aetiologic agent of dental caries, is a pathogen for infective endocarditis (IE). We investigated strains that express collagen-binding proteins (CBPs) with further classification based on expression of the 190-kDa protein antigen (PA).Method: Zeta-potential values of strains TW871 (CBP+/PA+) and MT8148 (CBP-/PA+), and their respective PA-defective mutant strains TW871PD (CBP+/PA-) and MT8148PD (CBP-/PA-), were analysed, as were their adhesion to and invasion of human umbilical vein endothelial cells (HUVECs). The distribution of strains from the oral cavities of 200 healthy individuals was analysed for CBP and/or PA expression and the strains were characterised for their adhesion and invasion properties.Results: TW871PD and MT8148PD showed significantly lower zeta-potential values than TW871 and MT8148, respectively. Collagen-binding rates were significantly higher for TW871PD than for TW871 but nearly negligible for MT8148 and MT8148PD. The adhesion and invasion rates of HUVECs were significantly higher for TW871PD than for TW871 and significantly higher for TW871 than for MT8148 and MT8148PD. The prevalence of CBP+ strains was similar to 10% and similar to 3% in the case of CBP+/PA- strains. Analyses of 200 clinical strains showed the CBP+/PA- group to have higher adhesion and invasion rates than other groups.Conclusions: CBP+/PA- S. mutans strains, despite their low distribution frequency, may be highly virulent for infective endocarditis. (C) 2013 Elsevier Ltd. All rights reserved.