p38 MAPK contributes to CD54 expression and the enhancement of phagocytic activity during macrophage development
p38 MAPK contributes to CD54 expression and the enhancement of phagocytic activity during macrophage development
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p38 MAPK 有助于 CD54 表达并增强巨噬细胞发育过程中的吞噬活性。
DOI：
10.1016/j.cellimm.2008.12.003
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Shen, Beifen
中科院分区：
文献类型：
--
作者：
Cui, Jian;Zhu, Ning;Shen, Beifen
p38 是丝裂原激活蛋白激酶 (MAPK) 超家族的一个亚家族，具有四种亚型。众所周知，p38 在炎症分子的产生中发挥着核心作用，因此是激活巨噬细胞响应炎症刺激所必需的。然而，人们对 p38 在巨噬细胞发育中的作用知之甚少。获得缺乏多种 p38 同工型的小鼠非常困难，这使得 p38 在巨噬细胞发育中的研究变得复杂。通过骨髓源性小鼠巨噬细胞模型和高选择性 p38 α/β 抑制剂 SB203580 和 SB239063，我们在此报告巨噬细胞集落刺激因子 (M-CSF) 在巨噬细胞发育过程中诱导 p38 激活。抑制 p38 活性对巨噬细胞增殖或存活影响较小，并且不会阻断 CD14、F4/80 的表达。然而，p38 抑制剂导致 CD54 表达显着减少，吞噬细胞活性受损。综上所述，我们的数据表明 p38 有助于巨噬细胞的发育。 (C) 2008 Elsevier Inc. 保留所有权利。
p38 is a subfamily of the mitogen-activated protein kinase (MAPK) superfamily with four isoforms. It has been well established that p38 plays a central role in the production of inflammatory molecules and is therefore required for the activation of macrophages in response to inflammatory stimuli. However, little is known about the roles of p38 in macrophage development. The difficulty to get mice deficient in multiple p38 isoforms complicates the study of p38 in macrophage development. With the model of bone marrow-derived murine macrophages and highly selective p38 alpha/beta inhibitors SB203580 and SB239063, here we report that macrophage colony-stimulating factor (M-CSF) induces p38 activation during macrophage development. Inhibition of p38 activity showed minor effects on macrophage proliferation or survival, and did not block CD14, F4/80 expression. However, p38 inhibitors resulted in a significant reduction in CD54 expression and impaired phagocytic activity. Taken together, our data suggest that p38 contributes to macrophage development. (C) 2008 Elsevier Inc. All rights reserved.