Rapid and Widespread Effects of 17β-Estradiol on Intracellular Signaling in the Male Songbird Brain: A Seasonal Comparison
Rapid and Widespread Effects of 17β-Estradiol on Intracellular Signaling in the Male Songbird Brain: A Seasonal Comparison
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DOI：
10.1210/en.2011-1525
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发表时间：
2012-03-01
期刊：
影响因子：
4.8
通讯作者：
Soma, Kiran K.
中科院分区：
文献类型：
--
作者：
Heimovics, Sarah A.;Prior, Nora H.;Soma, Kiran K.
在脊椎动物物种中，17 β-雌二醇（E-2）通过基因组和非基因组机制作用于大脑，影响神经元的生理和行为。非基因组E-2信号通常由膜相关雌激素受体启动，其调节细胞内信号级联，包括ERK的快速磷酸化。磷酸化的ERK（pERK）又可以迅速磷酸化酪氨酸羟化酶（TH）和cAMP反应元件结合蛋白（CREB）。最近的数据表明，E-2对小鼠攻击行为的快速影响在短光周期（冬季）期间更加突出，并且急性芳香化酶抑制仅在冬季降低鸣禽的攻击性。迄今为止，尚未研究E-2对细胞内信号传导的快速影响的季节性可塑性。在这里，我们比较了急性（15分钟）E-2治疗pERK，pTH和pCREB免疫反应性的雄性歌曲麻雀（Melospiza melodia）预处理芳香化酶抑制剂法倔唑在繁殖和非繁殖季节。我们检查了14个脑区的免疫反应性，包括部分的歌曲控制系统，社会行为网络和海马（Hp）。在这两个季节，E-2显着降低pERK在带核的杏仁核，pTH在腹内侧下丘脑，和pCREB在中脑中央灰质，坚固的Arcopallium核，和尾内侧巢皮质。然而，有几种影响严重依赖于季节。E-2仅在繁殖季节降低尾内侧巢皮质的pERK，仅在非繁殖季节降低内侧视前核的pCREB。在繁殖季节，E-2使Hp的pERK降低，而在非繁殖季节，E-2使Hp的pERK升高。总之，这些数据表明，E-2对男性大脑多个区域的细胞内信号传导具有快速影响，并且还表明E-2的快速影响在不同季节可能会有很大的不同。（内分泌学153：1364-1376，2012）
Across vertebrate species, 17 beta-estradiol (E-2) acts on the brain via both genomic and nongenomic mechanisms to influence neuronal physiology and behavior. Nongenomic E-2 signaling is typically initiated by membrane-associated estrogen receptors that modulate intracellular signaling cascades, including rapid phosphorylation of ERK. Phosphorylated ERK (pERK) can, in turn, rapidly phosphorylate tyrosine hydroxylase (TH) and cAMP response element-binding protein (CREB). Recent data suggest that the rapid effects of E-2 on mouse aggressive behavior are more prominent during short photoperiods (winter) and that acute aromatase inhibition reduces songbird aggression in winter only. To date, seasonal plasticity in the rapid effects of E-2 on intracellular signaling has not been investigated. Here, we compared the effects of acute (15 min) E-2 treatment on pERK, pTH, and pCREB immunoreactivity in male song sparrows (Melospiza melodia) pretreated with the aromatase inhibitor fadrozole during the breeding and nonbreeding seasons. We examined immunoreactivity in 14 brain regions including portions of the song control system, social behavior network, and the hippocampus (Hp). In both seasons, E-2 significantly decreased pERK in nucleus taeniae of the amygdala, pTH in ventromedial hypothalamus, and pCREB in mesencephalic central gray, robust nucleus of the arcopallium, and caudomedial nidopallium. However, several effects were critically dependent upon season. E-2 decreased pERK in caudomedial nidopallium in the breeding season only and decreased pCREB in the medial preoptic nucleus in the nonbreeding season only. Remarkably, E-2 decreased pERK in Hp in the breeding season but increased pERK in Hp in the nonbreeding season. Together, these data demonstrate that E-2 has rapid effects on intracellular signaling in multiple regions of the male brain and also demonstrate that rapid effects of E-2 can be profoundly different across the seasons. (Endocrinology 153: 1364-1376, 2012)