A role of jumonji gene in proliferation but not differentiation of megakaryocyte lineage cells
A role of jumonji gene in proliferation but not differentiation of megakaryocyte lineage cells
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DOI：
10.1016/s0301-472x(00)00686-x
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发表时间：
2001-04-01
影响因子：
2.6
通讯作者：
Takeuchi, T
中科院分区：
文献类型：
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作者：
Kitajima, K;Kojima, M;Takeuchi, T
Objective.在这项研究中，巨核细胞生成的隐性突变小鼠，jumonji，通过基因陷阱策略获得的调查。我们调查了巨核祖细胞的数量在胎肝，卵黄囊，外周血jumonji纯合子胚胎体外集落形成试验和监测集落形成从单个巨核祖细胞。我们还研究了巨核细胞分化标志物PF 4、CD 62 P和加塔-1的表达、前血小板形成、胞质成熟和胞内有丝分裂对jumonji缺陷型巨核细胞分化的影响。我们发现jumonji纯合子的胎粪、卵黄囊和外周血中巨核祖细胞的数量增加。与对照组相比，来自jumonji纯合子胎肝的一部分巨核祖细胞在体外形成了更大的集落。这种异常是由后代的延迟生长停滞引起的。未成熟的巨核祖细胞表现出这种异常。jumonji纯合子巨核细胞表达PF 4、CD 62 P和加塔-1，细胞质成熟，前血小板样突起延长，并发生内有丝分裂。jumonji基因的缺失导致巨核细胞谱系细胞数量的增加。我们的数据表明，jumonji基因调节巨核细胞系细胞的增殖，但不分化。(C)2001年国际实验血液学学会，Elsevier Science Inc.出版。
Objective. In this study, megakaryocytopoiesis was investigated in the recessive mutant mouse, jumonji, obtained by a gene-trap strategy.Materials and Methods. We investigated the number of megakaryocyte progenitors in the fetal liver, yolk sac, and peripheral blood of jumonji homozygous embryos by in vitro colony forming assay and monitored colony formation from single megakaryocyte progenitors. We also investigated the differentiation of jumonji-deficient megakaryocytes in terms of the expression of megakaryocyte differentiation markers PF4, CD62P, and GATA-1, proplatelet formation, cytoplasmic maturation, and endomitosis.Results. We found that the population of megakaryocyte progenitors in the fetal li c er, yolk sac, and peripheral blood of jumonji homozygotes increased. A fraction of megakaryocyte progenitors derived from the fetal liver of jumonji homozygotes formed larger colonies in vitro when compared with controls. This abnormality is caused by delayed growth arrest in the progeny. Immature megakaryocyte progenitors showed this abnormality. The megakaryocytes of jumonji homozygotes expressed PF4, CD62P, and GATA-1, obtained cytoplasmic maturation, extended proplatelet-like processes, and underwent endomitosis.Conclusion. The loss of the jumonji gene causes an increase in the number of megakaryocyte lineage cells. Our data suggest that the jumonji gene regulates proliferation but not differentiation of megakaryocyte lineage cells. (C) 2001 International Society for Experimental Hematology, Published by Elsevier Science Inc.