Monomeric rhodopsin is the minimal functional unit required for arrestin binding.
Monomeric rhodopsin is the minimal functional unit required for arrestin binding.
复制标题
单体视紫红质是抑制蛋白结合所需的最小功能单元。
DOI：
10.1016/j.jmb.2010.04.029
复制
发表时间：
2010
影响因子：
5.6
通讯作者：
Farrens,DavidL
中科院分区：
文献类型：
--
作者：
Tsukamoto,Hisao;Sinha,Abhinav;DeWitt,Mark;Farrens,DavidL
我们已经测试了抑制蛋白结合是否需要G蛋白偶联受体是二聚体或多聚体。为了做到这一点，我们将单视紫红质分子封装到纳米级磷脂颗粒（所谓的纳米盘）中，并测量它们结合arrestin的能力。我们的数据清楚地表明，视觉抑制蛋白和β-抑制蛋白1都可以与单体视紫红质结合，并稳定活性视紫红质II形式。有趣的是，我们发现，纳米盘中的单体视紫红质对野生型抑制蛋白结合的亲和力高于脂质体或纳米盘中的寡聚视紫红质，如通过稳定后视紫红质II所评估的。总之，这些结果确立了视紫红质自缔合不需要使抑制蛋白结合。
We have tested whether arrestin binding requires the G-protein-coupled receptor be a dimer or a multimer. To do this, we encapsulated single-rhodopsin molecules into nanoscale phospholipid particles (so-called nanodiscs) and measured their ability to bind arrestin. Our data clearly show that both visual arrestin and β-arrestin 1 can bind to monomeric rhodopsin and stabilize the active metarhodopsin II form. Interestingly, we find that the monomeric rhodopsin in nanodiscs has a higher affinity for wild-type arrestin binding than does oligomeric rhodopsin in liposomes or nanodiscs, as assessed by stabilization of metarhodopsin II. Together, these results establish that rhodopsin self-association is not required to enable arrestin binding.