DEMONSTRATION OF CORESISTANCE OF HAEMONCHUS-CONTORTUS TO IVERMECTIN AND MOXIDECTIN
DEMONSTRATION OF CORESISTANCE OF HAEMONCHUS-CONTORTUS TO IVERMECTIN AND MOXIDECTIN
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DOI：
10.1136/vr.132.26.651
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发表时间：
1993-06-26
影响因子：
2.2
通讯作者：
JOHNSON, SS
中科院分区：
文献类型：
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作者：
CONDER, GA;THOMPSON, DP;JOHNSON, SS
进行研究以确定对伊维菌素具有抗性的生物体是否也对莫昔克丁具有抗性。通过使用标准微电极技术测量莫昔克丁和伊维菌素在普通滨蟹（Pachygrapsus crassipes）腿部肌肉纤维中引起的膜电导变化，比较了莫昔克丁和伊维菌素的作用机制。爪沙鼠 (Jirds) 被伊维菌素耐药或敏感的捻转血矛线虫菌株感染，用莫昔克丁或伊维菌素处理，剂量通常可以清除 98% 以上的敏感菌株，并检查寄生虫清除情况。结果表明，莫昔克汀会导致肌肉制剂中的膜抵抗力迅速丧失，而氯离子通道阻滞剂印克拉毒素的效果几乎可以逆转 50%；这种活性模式在性质上与伊维菌素相似。在 Jird 模型中，莫昔克丁在一份救济金下对伊维菌素耐药菌株的清除率仅为小于或等于 47.2%，而对伊维菌素敏感菌株的清除率总是大于或等于 47.2%。这些结果表明莫西菌素和伊维菌素具有共同的作用机制，对伊维菌素具有抗药性的生物体也可能对莫西菌素具有抗药性。
Studies were conducted to determine whether organisms which are resistant to ivermectin are also resistant to moxidectin. The mechanisms of action of moxidectin and ivermectin were compared by measuring the changes in membrane conductance they induced in leg muscle fibres of the common shore crab (Pachygrapsus crassipes) by using standard micro-electrode techniques. Meriones unguiculatus (Jirds) were infected with ivermectin-resistent or -susceptible strains of Haemonchus contortus, treated with moxidectin or ivermectin at a dose which routinely clears more than 98 per cent of the susceptible strain, and examined for parasite clearance. The results showed that moxidectin induced a rapid loss of membrane resistance in the muscle preparation, and that the effect was almost 50 per cent reversible with the chloride channel-blocker picratoxinin; this pattern of activity is qualitatively similar to that of ivermectin. In the Jird model, moxidectin achieved a clearance of only less-than-or-equal-to 47.2 per cent against ivermectin-resistant H contortus at a dole which invariably clears greater-than-or-equal-to per cent of an ivermectin-susceptible strain. These results indicate that moxidectin and ivermectin share a common mechanism of action, and that organisms that are resistant to ivermectin are also likely to be resistant to moxidectin.