A toll receptor and a cytokine, Toll5A and Spz1C, are involved in toll antifungal immune signaling in the mosquito Aedes aegypti
A toll receptor and a cytokine, Toll5A and Spz1C, are involved in toll antifungal immune signaling in the mosquito Aedes aegypti
复制标题
DOI：
10.1074/jbc.m608912200
复制
发表时间：
2006-12-22
影响因子：
4.8
通讯作者：
Raikhel, Alexander S.
中科院分区：
文献类型：
--
作者：
Shin, Sang Woon;Bian, Guowu;Raikhel, Alexander S.
埃及伊蚊的真菌特异性免疫反应涉及通过 REL1 转导的 Toll 免疫途径，REL1 是 NF-κ B 转录因子果蝇 Dorsal 的同源物。 Toll 受体及其配体 Spatzle (Spz) 将细胞外免疫信号与 Toll 细胞内转导途径连接起来。从基因组和 cDNA 序列数据库中鉴定出果蝇 Toll (Toll1) 受体的 5 个同源物（Toll1A、Toll1B、Toll5A、Toll5B 和 Toll4）以及果蝇细胞因子 Spatzle 的 3 个同源物（Spz1A、1B 和 1C）。 Toll1A、Toll5A、Toll5B 和 Spz1A 在真菌攻击后在蚊子脂肪体中被特异性诱导。这种转录上调是由 REL1 介导的。 Spz1C在蚊子脂肪体中组成型表达，而Spz1B和Toll4主要在雌性蚊子的卵巢组织中表达。 Toll1B 的转录本仅在蚊子胚胎的早期阶段被检测到。 RNA 干扰敲低 Toll5A 和 Spz1C 导致伊蚊 Toll/REL1 通路缺陷的两种表型：真菌攻击后伊蚊 Serpin-27A 的诱导减少，以及对昆虫病原真菌白僵菌的易感性增加。这些数据表明，Toll5A 和 Spz1C 作为细胞因子受体系统，专门针对蚊子脂肪体中真菌攻击后 Toll 受体介导的免疫反应。
The fungal-specific immune response in the mosquito Aedes aegypti involves the Toll immune pathway transduced through REL1, a homologue of the NF-kappa B transcription factor Drosophila Dorsal. The Toll receptor and its ligand, Spatzle (Spz), link extracellular immune signals to the Toll intracellular transduction pathway. Five homologues to the Drosophila Toll (Toll1) receptor (Toll1A, Toll1B, Toll5A, Toll5B, and Toll4) and three homologues to the Drosophila cytokine Spatzle (Spz1A, 1B, and 1C) were identified from genomic and cDNA sequence data bases. Toll1A, Toll5A, Toll5B, and Spz1A were specifically induced in the mosquito fat body following fungal challenge. This transcriptional up-regulation was mediated by REL1. Spz1C was constitutively expressed in the mosquito fat body, whereas Spz1B and Toll4 were primarily expressed in ovarian tissues of female mosquitoes. The transcripts of Toll1B were only detected in early stages of mosquito embryos. RNA interference knock down of Toll5A and Spz1C resulted in two phenotypes of Aedes Toll/REL1 pathway deficiency: decreased induction of Aedes Serpin-27A following fungal challenge and increased susceptibility to the entomopathogenic fungus Beauveria bassiana. These data suggest that Toll5A and Spz1C function as cytokine receptor systems specific to the Toll receptor-mediated immune response following fungal challenge in the mosquito fat body.