Mutagenicity profile of atmospheric particulate matter in a small urban center subjected to airborne emission from vehicle traffic and sugar cane burning
Mutagenicity profile of atmospheric particulate matter in a small urban center subjected to airborne emission from vehicle traffic and sugar cane burning
复制标题
小型城市中心大气颗粒物受车辆交通和甘蔗燃烧产生的空气排放影响的致突变性概况
DOI：
10.1002/em.21970
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发表时间：
2016
影响因子：
2.8
通讯作者：
G. Umbuzeiro
中科院分区：
文献类型：
--
作者：
D. K. Alves;Fábio Kummrow;A. Cardoso;D. A. Morales;G. Umbuzeiro
大气颗粒物（PM）具有遗传毒性，最近被国际癌症研究机构列为对人类致癌物。PM的化学成分因来源和大气条件而异。沙门氏菌/微粒体试验是最常用的致突变性试验，可鉴别导致观察到的致突变性的主要化学类别。这项工作的目的是表征的致突变性的PM样品从农村城市，Limeira，巴西，这是受交通繁忙和甘蔗生物质燃烧。收集了6个总PM样品。计算了气团后向轨迹。在存在和不存在代谢活化（S9）的情况下，使用TA 98、YG 1041和TA 1538，通过沙门氏菌/微粒体微悬浮液致突变性试验对有机提取物进行了分析。YG 1041是最敏感的菌株，在没有代谢活化的情况下，致突变性达到9，700个回复突变体/m3。TA 1538的效价高于TA 98，表明在空气致突变性研究中应考虑该菌株。相对于TA 98，对YG 1041的响应增加，而对S9的响应降低，表明硝基芳烃是主要贡献者。利梅拉是世界上最具诱变性的城市之一。当甘蔗生产地区的空气与受人为活动（如交通）影响的地区的空气混合时，似乎会在Limeira产生高致突变性。硝基多环芳烃的形成增加可能是由于芳香化合物与高NOx和臭氧浓度的大气之间接触时间较长，尽管需要更多的研究来证实这一假设。Environ.摩尔变异体57：41-50，2016.© 2015威利期刊公司.
Atmospheric particulate matter (PM) is genotoxic and recently was classified as carcinogenic to humans by the International Agency for Research on Cancer. PM chemical composition varies depending on source and atmospheric conditions. The Salmonella/microsome assay is the most used mutagenicity test and can identify the major chemical classes responsible for observed mutagenicity. The objective of this work was to characterize the mutagenicity of PM samples from a countryside city, Limeira, Brazil, which is influenced by heavy traffic and sugar cane biomass burning. Six samples of total PM were collected. Air mass backward trajectories were calculated. Organic extracts were assayed using the Salmonella/microsome microsuspension mutagenicity assay using TA98, YG1041, and TA1538, with and without metabolic activation (S9). YG1041 was the most sensitive strain and mutagenicity reached 9,700 revertants per m3 without metabolic activation. Potency for TA1538 was higher than TA98, indicating that this strain should be considered in air mutagenicity studies. The increased response to YG1041 relative to TA98, and the decreased response with S9, suggests that nitroaromatics are the major contributors. Limeira is among the most mutagenic cities in the world. High mutagenicity in Limeira seems to occur when the air mass from the area of sugarcane production is mixed with air from the region impacted by anthropogenic activities such as traffic. An increase in the formation of nitro‐polycyclic aromatic hydrocarbons may result from longer contact time between the aromatic compounds and the atmosphere with high NOx and ozone concentration, although more studies are required to confirm this hypothesis. Environ. Mol. Mutagen. 57:41–50, 2016. © 2015 Wiley Periodicals, Inc.